胡志刚, 博士，教授
南京师范大学生命科学学院
江苏省分子医学生物技术重点实验室
工作学习经历：
2022年7月至今，南京师范大学生命科学学院 教授
2014年1月至2022年7月，南京师范大学生命科学学院 副教授
2010年1月至2013年11月，斯坦福大学医学院博士后
2007年2月至2009年，韩国东亚大学自然资源与生命科学学院，理学博士学位
研究方向：
1、DNA损伤修复与肿瘤发生研究
2、脂代谢相关研究
3、CAR-T等肿瘤免疫治疗
主持科研项目：
1、DNA聚合酶β在肥胖相关脂质代谢紊乱中的作用及机制研究，2022.01-2025.12，国家自然科学基金面上项目
2、GIPC1/SR-B1调节体内胆固醇平衡和脂代谢研究，2019.7-2022.6，江苏省教育厅重点项目
3、高密度脂蛋白受体（SR-BI）的泛素化修饰及功能研究，2015.01-2017.12，国家自然科学基金青年项目
4、高密度脂蛋白受体（SR-BI）蛋白质翻译后修饰及功能研究，2014.07-2017.06，江苏省自然科学基金青年项目
5、高密度脂蛋白磷酸化修饰及功能研究，2014.08-2016.12，江苏省高校自然科学研究面上项目
6、miR-24对高密度脂蛋白受体(SR-BI)的表达调控及机制研究，教育部留学回国人员科研项目启动基金
主持教改项目：
1、南京师范大学2022年创新性实验教学项目，基于SDS-PAGE电泳的蛋白质差异表达分析
2、南京师范大学2021年度“课程思政”建设示范课程，分子生物学
3、2020年度南京师范大学高等教育改革研究课题，基于慕课和雨课堂等网络教学资源和工具的分子生物学课程教学改革与实践
4、2015年南京师范大学教育教改研究课题，“发现式”教学在细胞生物学课程中的应用
学术兼职：
江苏省生理科学学会秘书长；中国细胞生物学会会员；中国生物化学与分子生物学学会会员，江苏省生物化学与分子生物学青年委员会委员；Cells, Cancers, Frontier in endocrinology, Acta Biochimica et Biophysica Sinica等期刊审稿人；教育部学位与研究生教育发展中心学位论文评审专家。
荣誉奖励：
2022年：南京师范大学2021年度本科优秀教学 一等奖
全国大学生生命科学竞赛（科学探索类）国家三等奖 指导教师
全国大学生生命科学竞赛（创新创业类）国家三等奖 指导教师
南京师范大学优秀本科生毕业论文（王淑妍） 指导教师
南京师范大学优秀学术学位硕士学位论文（李恩洁） 指导教师
2021年：江苏省高等学校科学技术研究成果奖一等奖（排名第三）
南京师范大学2020年度本科优秀教学 二等奖
全国大学生生命科学竞赛（科学探索类）国家二等奖 指导教师
全国大学生生命科学竞赛（创新创业类）国家一等奖 指导教师
南京师范大学优秀本科生毕业论文（徐怡华） 指导教师
2020年：第五届全国大学生生命科学创新创业大赛优秀成果奖三等奖 指导教师
江苏省生物化学与分子生物学学会年度单项奖
2019年：2019年江苏省“郑集青年科技论文奖”三等奖（刘蕊）指导教师
2019年江苏省科学技术协会青年创新创业大赛优秀奖（排名第一）
全国大学生生命科学竞赛江苏省赛区三等奖 指导教师
Email: huzg_2000AT126.com (发邮件时请将AT换成@)
代表性论著(*通讯作者)：
1. Chen J, Hu J, Gu L, Ji F, Zhang F, Zhang M, Li J, Chen Z, Jiang L, Zhang Y, Shi R, Ma L, Jia S, Zhang Y, Zhang Q, Liang J, Yao S, Hu Z*, Guo Z*. Anti-mesothelin CAR-T immunotherapy in patients with ovarian cancer. Cancer Immunol Immunother. 2023 Feb;72(2):409-425.
2. Li E, Xia M, Du Y, Long K, Ji F, Pan F, He L, Hu Z*, Guo Z*. METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis. Elife. 2022 May 3;11:e75231.
3. Ji F, Zhang F, Zhang M, Long K, Xia M, Lu F, Li E, Chen J, Li J, Chen Z, Jing L, Jia S, Yang R, Hu Z*, Guo Z*. Targeting the DNA damage response enhances CD70 CAR-T cell therapy for renal carcinoma by activating the cGAS-STING pathway. J Hematol Oncol. 2021 Sep 23;14(1):152.
4. Long K, Gu L, Li L, Zhang Z, Li E, Zhang Y, He L, Pan F, Guo Z*, Hu Z*. Small-molecule inhibition of APE1 induces apoptosis, pyroptosis, and necroptosis in non-small cell lung cancer. Cell Death Dis. 2021 May 18;12(6):503.
5. Zhang Z, Zhou Q, Liu R, Liu L, Shen WJ, Azhar S, Qu YF, Guo Z, Hu Z*. The adaptor protein GIPC1 stabilizes the scavenger receptor SR-B1 and increases its cholesterol uptake. J Biol Chem. 2021 Jan-Jun;296:100616.
6. Wang M, Long K, Li E, Li L, Li B, Ci S, He L, Pan F, Hu Z*, Guo Z*. DNA polymerase beta modulates cancer progression via enhancing CDH13 expression by promoter demethylation. Oncogene. 2020 Aug;39(33):5507-5519.
7. Lu X, Liu R, Wang M, Kumar AK, Pan F, He L, Hu Z*, Guo Z*. MicroRNA-140 impedes DNA repair by targeting FEN1 and enhances chemotherapeutic response in breast cancer. Oncogene. 2020 Jan;39(1):234-247.
其他论文(*通讯作者)：
8. Du Y, Xia M, Hu Z*. Analysis of N 6 -methyladenosine RNA Modification Levels by Dot Blotting. Bio Protoc. 2022 Dec 5;12(23):e4565.
9. Xia M, Wang S, Qi Y, Long K, Li E, He L, Pan F, Guo Z, Hu Z*. Inhibition of O-GlcNAc transferase sensitizes prostate cancer cells to docetaxel. Front Oncol. 2022 Nov 10;12:993243.
10. Yang F, Hu Z*, Guo Z*. Small-Molecule Inhibitors Targeting FEN1 for Cancer Therapy. Biomolecules. 2022 Jul 20;12(7):1007.
11. Wang L, Chen G, Wu S, Xu Y, Guo C, Wang M, Liang T, Guo Z, Di HJ*, Hu Z*. Genipin improves lipid metabolism and sperm parametersin obese mice via regulation of miR-132 expression. Acta Biochim Biophys Sin (Shanghai). 2022 Aug 25. doi: 10.3724/abbs.2022120. Epub ahead of print. PMID: 36082932.
12. Yang F, Hu Z*, Guo Z*. Small-Molecule Inhibitors Targeting FEN1 for Cancer Therapy. Biomolecules. 2022 Jul 20;12(7):1007.
13. Li M, Xia M, Zhang Z, Tan Y, Li E, Guo Z, Fang M, Zhu Y*, Hu Z*. METTL3 antagonizes 5 FU chemotherapy and confers drug resistance in colorectal carcinoma. Int J Oncol. 2022 Sep;61(3):106.
14. Gu L, Sun Y, Wu T, Chen G, Tang X, Zhao L, He L, Hu Z, Sun L, Pan F, Yin Z, Guo Z. A novel mechanism for macrophage pyroptosis in rheumatoid arthritis induced by Pol β deficiency. Cell Death Dis. 2022 Jul 6;13(7):583.
15. Xia M, Guo Z*, Hu Z*. The Role of PARP Inhibitors in the Treatment of Prostate Cancer: Recent Advances in Clinical Trials. Biomolecules. 2021 May 12;11(5):722.
16. Li L, Kumar AK, Hu Z*, Guo Z*. Small Molecule Inhibitors Targeting Key Proteins in the DNA Damage Response for Cancer Therapy. Curr Med Chem. 2021;28(5):963-985..
17. Wang W, Ma Y, Huang M, Liang W, Zhao X, Li Q, Wang S, Hu Z, He L, Gao T, Chen J, Pan F, Guo Z. Asymmetrical arginine dimethylation of histone H4 by 8-oxog/OGG1/PRMT1 is essential for oxidative stress-induced transcription activation. Free Radic Biol Med. 2021 Feb 20;164:175-186.
18. Wu T, Zhu H, Zhang M, Sun Y, Yang Y, Gu L, Zhang J, Mu D, Wu C, Hu Z, Jiang L, Jia S, Zhang Y, He L, Pan FY, Guo Z. FEN1 inhibitor synergizes with low-dose camptothecin to induce increased cell killing via the mitochondria mediated apoptotic pathway. Gene Ther. 2021 Jan 7.
19. Xu Y, Wang L, Cao S, Hu R, Liu R, Hua K, Guo Z, Di HJ*, Hu Z*. Genipin improves reproductive health problems caused by circadian disruption in male mice. Reprod Biol Endocrinol. 2020 Dec 11;18(1):122.
20. Hua K, Wang L, Sun J, Zhou N, Zhang Y, Ji F, Jing L, Yang Y, Xia W, Hu Z, Pan F, Chen X, Yao B, Guo Z. Impairment of Pol β-related DNA base-excision repair leads to ovarian aging in mice. Aging (Albany NY). 2020 Nov 20;12(24):25207-25228.
21. He L#, Hu Z#, Sun Y#, Zhang M, Zhu H, Jiang L, Zhang Q, Mu D, Zhang J, Gu L, Yang Y, Pan FY, Jia S, Guo Z. PRMT1 is critical to FEN1 expression and drug resistance in lung cancer cells. DNA Repair (Amst). 2020 Nov;95:102953.
22. Ma Z, Wang W, Wang S, Zhao X, Ma Y, Wu C, Hu Z, He L, Pan F, Guo Z. Symmetrical dimethylation of H4R3: A bridge linking DNA damage and repair upon oxidative stress. Redox Biol. 2020 Oct;37:101653.
23. Zhang J, Jing L, Tan S, Zeng EM, Lin Y, He L, Hu Z, Liu J, Guo Z. Inhibition of miR-1193 leads to synthetic lethality in glioblastoma multiforme cells deficient of DNA-PKcs. Cell Death Dis. 2020 Jul 30;11(7):602.
24. Ci S, Xia W, Liang W, Qin L, Zhang Y, Dianov GL, Wang M, Zhao X, Wu C, Alagamuthu KK, Hu Z, He L, Pan F, Guo Z. Src-mediated phosphorylation of GAPDH regulates its nuclear localization and cellular response to DNA damage. FASEB J. 2020 Aug;34(8):10443-10461.
25. Liu R, Wang M, Li E, Yang Y, Li J, Chen S, Shen WJ, Azhar S, Guo Z, Hu Z*. Dysregulation of microRNA-125a contributes to obesity-associated insulin resistance and dysregulates lipid metabolism in mice. Biochim Biophys Acta-Mol Cell Biol Lipids. 2020 May;1865(5):158640.
26. Azhar S, Dong D, Shen WJ, Hu Z, Kraemer FB. The role of miRNAs in regulating adrenal/gonadal steroidogenesis. J Mol Endocrinol. 2020 Jan;64(1):R21-R43.
27. Zhu H, Wu C, Wu T, Xia W, Ci S, He W, Zhang Y, Li L, Zhou S, Zhang J, Edick AM, Zhang A, Pan FY, Hu Z, He L, Guo Z. Inhibition of AKT Sensitizes Cancer Cells to Antineoplastic Drugs by Downregulating Flap Endonuclease 1. Mol Cancer Ther. 2019 Dec;18(12):2407-2420.
28. Wang M, Li E, Lin L, Kumar AK, Pan F, He L, Zhang J, Hu Z*, Guo Z*. Enhanced Activity of Variant DNA Polymerase β (D160G) Contributes to Cisplatin Therapy by Impeding the Efficiency of NER. Mol Cancer Res. 2019 Oct;17(10):2077-2088.
29. Xia W, Ci S, Li M, Wang M, Dianov GL, Ma Z, Li L, Hua K, Alagamuthu KK, Qing L, Luo L, Edick AM, Liu L, Hu Z, He L, Pan F, Guo Z. Two-way crosstalk between BER and c-NHEJ repair pathway is mediated by Pol-β and Ku70. FASEB J. 2019 Nov;33(11):11668-11681.
30. He L, Zhu H, Zhou S, Wu T, Wu H, Yang H, Mao H, SekharKathera C, Janardhan A, Edick AM, Zhang A, Hu Z, Pan F, Guo Z. Wnt pathway is involved in 5-FU drug resistance of colorectal cancer cells. Exp Mol Med. 2018 Aug 14;50(8):101.
31. Wang M, Li L, Liu R, Song Y, Zhang X, Niu W, Kumar AK, Guo Z, Hu Z*. Obesity-induced overexpression of miRNA-24 regulates cholesterol uptake and lipid metabolism by targeting SR-B1. Gene. 2018 Aug 20; 668:196-203.
32. He L, Yang H, Zhou S, Zhu H, Mao H, Ma Z, Wu T, Kumar AK, Kathera C, Janardhan A, Pan F, Hu Z, Yang Y, Luo L, Guo Z. Synergistic antitumor effect of combined paclitaxel with FEN1 inhibitor in cervical cancer cells. DNA Repair. 2018 Mar;63:1-9.
33. He L, Luo L, Zhu H, Yang H, Zhang Y, Wu H, Sun H, Jiang F, Kathera CS, Liu L, Zhuang Z, Chen H, Pan F, Hu Z, Zhang J, Guo Z.. FEN1 promotes tumor progression and confers cisplatin resistance in non‐small cell lung cancer[J]. Molecular Oncology, 2017，11(9):1302-1303.
34. Hu Z, Shen WJ, et al. Regulation of adrenal and ovarian steroidogenesis by miR-132.[J]. Journal of Molecular Endocrinology, 2017, 59(3):269-283.
35. Lu X, He L, Zhou Q, Wang M, Shen WJ, Azhar S, Pan F, Guo Z, Hu Z*. NHERF1 and NHERF2 regulation of SR-B1 stability via ubiquitination and proteasome degradation.[J]. Biochemical & Biophysical Research Communications, 2017，490(4): 1168-1175.
36. Li J, Zhou Q, Ma Z, Wang M, Shen WJ, Azhar S, Guo Z, Hu Z*. Feedback inhibition of CREB signaling by p38 MAPK contributes to the negative regulation of steroidogenesis[J]. Reproductive Biology and Endocrinology, 2017, 15(1): 19.
37. Sun, H., He, L., Wu, H., Pan, F., Wu, X., Zhao, J., Hu, Z., Sekhar, C., Li, H., Zheng, L., Chen, H., Shen, B. H. and Guo, Z. (2017) The FEN1 L209P mutation interferes with long-patch base excision repair and induces cellular transformation. Oncogene. 36, 194-207.
38. Pan F, Zhao J, Zhou T, Kuang Z, Dai H, Wu H, Sun H, Zhou X, Wu X, Hu Z, He L, Shen B, Guo Z. Mutation of DNA Polymerase β R137Q Results in Retarded Embryo Development Due to Impaired DNA Base Excision Repair in Mice. Sci Rep. 2016, 6:28614.
39. Hu Z*, Li J, Kuang Z, et al. Cell-Specific Polymorphism and Hormonal Regulation of DNA Methylation in Scavenger Receptor Class B, Type I[J]. DNA and cell biology, 2016, 35(6): 280-289.
40. Zhou X, Zhuang Z, Wang W, He L, Wu H, Cao Y, Pan F, Zhao J, Hu Z, Sekhar C, Guo Z. OGG1 is essential in oxidative stress induced DNA demethylation. Cell Signal. 2016, 28(9):1163-1171.
41. Shen W J, Hu Z, Hu J, et al. Post-transcriptional and Post-translational Regulation of Steroidogenesis[M]//Post-transcriptional Mechanisms in Endocrine Regulation. Springer International Publishing, 2016: 253-275.
42. Zhou T, Pan F, Cao Y, Han Y, Zhao J, Sun H, Zhou X, Wu X, He L, Hu Z, Chen H, Shen B, Guo Z.. R152C DNA Pol β mutation impairs base excision repair and induces cellular transformation. Oncotarget, 2016, 7(6): 6902.
43. Hu Z, Hu J, Shen W J, et al. A Novel Role of Salt-Inducible Kinase 1 (SIK1) in the Post-Translational Regulation of Scavenger Receptor Class B Type 1 Activity. Biochemistry, 2015, 54(46):6917-30.
44. Cao Y, .. Hu Z.., Guo Z. Selection and characterization of human PCSK9 antibody from phage displayed antibody library[J]. Biochemical and biophysical research communications, 2015, 463(4): 712-718.
45. Shen W J, Hu J, Hu Z, et al. Scavenger receptor class B type I (SR-BI): a versatile receptor with multiple functions and actions[J]. Metabolism, 2014, 63(7): 875-886.
46. Hu Z, Shen W J, Cortez Y, et al. Hormonal regulation of microRNA expression in steroid producing cells of the ovary, testis and adrenal gland[J]. PloS one, 2013, 8(10): e78040-e78040.
47. Hu, Z., Hu, J., Zhang, Z., Shen, W. J., Yun, C. C., Berlot, C. H., ... & Azhar, S. (2013). Regulation of Expression and Function of Scavenger Receptor Class B, Type I (SR-BI) by Na+/H+ Exchanger Regulatory Factors (NHERFs). Journal of Biological Chemistry, 288(16), 11416-11435.
48. Hu, Z, et al. MicroRNAs 125a and 455 Repress Lipoprotein-Supported Steroidogenesis by Targeting Scavenger Receptor Class B Type I in Steroidogenic Cells. Molecular and Cellular Biology, 2012, 32(24): 5035-5045.
49. Hu Z., Lee K.S., et al. Molecular characterization of 1-Cys and 2-Cys peroxiredoxins from the bumblebee Bombus ignitus. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2010, 155(3): 272-280
50. Hu Z. et al. Molecular characterization of a phospholipid hydroperoxide glutathione peroxidase from the bumblebee Bombus ignitus. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2010, 155(1): 54-61
51. Xin Y., Choo Y.M., Hu Z., et al. Molecular cloning and characterization of a venom phospholipase A(2) from the bumblebee Bombus ignitus. Comp Biochem Physiol B Biochem Mol Biol. 2009, 154(2): 195-202.
52. Hu Z., & Sohn, H. D. (2009). Molecular Cloning and Characterization of a Lipocalin in the Bumblebee Bombus Ignitus. International Journal of Industrial Entomology, 19(2), 229-235.
53. Hu Z., et al. Molecular Cloning and Characterization of a Muscle-Specific Lipase from the Bumblebee Bombus ignitus. International Journal of Industrial Entomology, 2008, 17(1): 143-151.
54. Zhao Y., Yao Q., Tang Xu., Wang Q., Hu Z., et al. Cloning and homologic analysis of Tpn I gene in silkworm bombyx mori, African Journal of Biotechnology, 2007, 6(6): 672-676.
55. Hu Z., et al. Cloning and Characterization of Bombyx mori PP-BP a Gene Induced by Viral Infection, Acta Genetica Sinica, 2006, 33(11): 975-983.
56. Hu Z., Chen K., Gao L., Yao Q. RNA Interference to Prevent Bombyx mori Nuclear Polyhedrosis Virus Infection in Vivo, IJIE, 2006, 12(1):15-19.
57. Yao Q., Gao L., Chen K., Hu Z. Detection of proliferation of Bombyx mori nucleopolyhedrovirus in its host by fluorescence quantitative PCR. Acta entomological sinica. 2005，48(6)：871-875. In Chinese.
58. Hu Z. et al. Identification and characterization of Bombyx mori eIF5A gene through bioinformatics approaches, In Silico Biology, 2005, 5: 0051.
59. Yao Q., Hu Z., Chen K. Cloning and Characterization of Ribosome-associated Membrane Protein 4 (RAMP4) gene in silkworm Bombyx mori, International Journal of Industrial Entomology, 2005, 10(2):125-129.
