Dr. Tao Gao, Nanjing Normal University
【个人简介 / Profile】
高涛，博士，副教授，兼聘研究员。于2016年6月获南京大学生物学博士学位，同年8月入选全国“博士后创新人才支持计划”，2019年入职南京师范大学，同年入选江苏省“双创博士”、南京师范大学“中青年领军人才”，2021年入选江苏省“双创团队”核心成员，主持国家级和省部级项目5项，参编Springer、Elsevier专著6部/章节，发表SCI期刊论文70余篇，包括Nat. Commun.、CCS Chem.、Cell Commun. Signal.、Nano Lett.、Small、Anal. Chem.、Biosens. Bioelectron.、Sens. Actuator B-Chem.、TrAC-Anal. Chem.等期刊。
【链接 / Links】
Web Page: https://sky.njnu.edu.cn/info/1485/26241.htm
ResearchGate: https://www.researchgate.net/profile/Tao_Gao16
【研究领域 / Research Area】
生物电化学与生物医学测量
Bioelectrochemistry, Bioelectronic Devices & Measurements
【研究内容 / Research Interests】
以生命体系中的电活性分子、电子传递链、电活性细胞等作为研究对象，解析生命体系的质荷传输及其反应机理，探究跨“生物-器件”微纳界面的质荷传输机制和特性，从而构建生物电子器件与微纳功能界面，进行生物活体的信号获取和功能调控研究，为面向生命健康的生物电子元件、生物传感器和医学诊疗技术开发提供跨学科理论基础与技术创新。
（1）生物单分子的电化学测量，生物电子器件与传感界面的设计与构建；
（2）疾病分子靶标精准筛查，医学检验新技术、新方法与生物医学传感。
By using electroactive molecules, electron transport chains, and electroactive cells from biological systems, our studies elucidate fundamental bio-electrochemistry mechanisms: mass-charge transport and associated reaction processes, thus to investigate the mass-charge transfer acrossing bio/device interfaces at the “micro-nano” scales, aiming to construct advanced bioelectronic devices that encompasses both signal acquisition and functional regulation of living systems. We are trying to establish theoretical foundations and technological innovations for developing bioelectronic components, biosensors, and medical diagnostic/therapeutic technologies, ect. This interdisciplinary investigation bridges biological systems with electronic engineering, and may provide critical insights for next-generation innovations.
（1） Electrochemical measurements of single biomolecules
（2） Bioelectronic devices, biosensing surface and biosensors
（3） Analytical methods and devices for biomedical applications
【教育与研究经历 / Experience】
2025-12 至 今, 南京大学, 生命分析化学全国重点实验室, 固定成员
2023-03 至 今, 南京师范大学-鼓楼医院生命健康研究院, 兼聘研究员
2019-07 至 今, 南京师范大学, 生命科学学院, 副教授
2016-09 至 2018-08, 上海大学, 生物医学工程系, 博士后/讲师
2011-09 至 2016-06, 南京大学, 医药生物技术全国重点实验室, 博士
【论著、专利 / Publications & Patents】
一、生物医学传感（Biomedical Measurements & Laboratory Medicine）
1. Fang, X.; Zheng, J.; Gao, W.; Liu, Z.; Li, X.; Li, C.*; Gao, T.*; Zhang, R.*; Programmable nucleic acid fibers: Molecular weaving, dynamic functional engineering, and precision biomedical applications, Coord. Chem. Rev., 2025, 552: 217525.
2. Xie, H.; Wang, L.; Wang, G.; Deng, Y.; Hu, X.; Lin, Y.; Zhang, D.; Gao, T.*; Xie, P.*; Li, G.*; Preparation of living material with metal-organic frameworks and bacteria for the design of electrochemical biosensor to detect exosomes, Small, 2025, 21: e08741.
3. Cui, W.; Fang, X.; Zhang, Z.; Zhang, R.; Huang, Q.; Lu, R.; Gao, T.*; Tong, H. H. Y.*; Zheng, J.*; Efficient colorimetric assay promotes the virtual screen of granzyme b inhibitors, Microchim Acta, 2025, 192: 578.
4. Wang, L.; Xie, H.; Zhou, T.; Wang, M.; Yang, J.*; Gao, T.*; Li, G.*; Assembly of dye molecules in covalent organic frameworks for enhanced colorimetric biosensing, Anal. Chem., 2024, 96: 15720-15727.
5. Wang, H.; Fang, T.; Wang, J.; Zhang, M.; Mu, X.; Gao, T.*; Wei, T.*; Dai, Z.*; Adaptive size evolution of an MOFs-in-MOF nanovehicle for enhanced nucleus-targeted tumor chemotherapy, Nano Lett., 2024, 24: 10605-10613.
6. Xie, H.; Wang, L.; Yu, X.; Zhou, T.; Wang, M.; Yang, J.; Gao, T.*; Li, G.*; Synthesis of a COF-on-MOF hybrid nanomaterial for enhanced colorimetric biosensing, Talanta, 2024, 274: 126071.
7. Zhang, Y.; Xu, Q.; Wang, F.; Gao, T.*; Wei, T.*; Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites, Sens. Actuator B-Chem., 2023, 375: 132942.
8. Gao, T.; Zhao, S.; Sun, J.; Huang, Q.; Long, S.; Lv, M.; Ma, J.; Guo, Z.*; Li, G.*; Single-cell quantitative phenotyping via the aptamer-mounted Nest-PCR (Apt-nPCR), Anal. Chem., 2022, 94: 2383-2390.
9. Wang, X.; Gao, T.*; Zhu, J.; Long, S.; Zhao, S.; Yuan, L.*; Wang, Z.*; Fabrication of channeled and three-dimensional electrodes for the integrated capture and detection of invasive circulating tumor cells during hematogenous metastasis, Anal. Chem., 2023, 95: 2496-2503.
10. Wang, X.; Zhao, S.; Wang, Z.*; Gao, T.*; Platelets involved tumor cell EMT during circulation: Communications and interventions, Cell Commun Signal, 2022, 20: 82.
11. Wang, L.; Li, H.; Shi, L.; Li, L.; Jia, F.; Gao, T.*; Li, G.*; In situ peptide self-assembly on ionic nanochannel for dynamic monitoring of MMPs in extracellular matrix, Biosens. Bioelectron., 2022, 195: 113671.
12. Zeng, Y.; Gao, T.*; Li, G.*; Molecular modification and assembly at cell surface for biochemical analysis, SCIENTIA SINICA Vitae, 2021, 52: 373-386.
13. Wang, W. T.; Ma, Y.; Huang, M. L.; Liang, W. C.; Zhao, X. Q.; Li, Q. W.; Wang, S. W.; Hu, Z. G.; He, L. F.; Gao, T.; Chen, J. F.; Pan, F. Y.*; Guo, Z. G.*; Asymmetrical arginine dimethylation of histone H4 by 8-oxog/OGG1/PRMT1 is essential for oxidative stress-induced transcription activation, Free Radic. Biol. Med., 2021, 164: 175-186.
14. Shi, L.; Wang, L.; Ma, X.; Jalalah, M.; Alsareii, S. A.; Gao, T.*; Harraz, F. A.*; Li, G.*; Electrochemical trans-channel assay for efficient evaluation of tumor cell invasiveness, ACS Appl Mater Interfaces, 2021, 13: 17268-17275.
15. Yang, Y.; He, M.; Wei, T.; Sun, J.; Wu, S.; Gao, T.*; Guo, Z.*; Photo-affinity pulling down of low-affinity binding proteins mediated by post-translational modifications, Anal. Chim. Acta, 2020, 1107: 164-171.
16. Gao, T.; Li, L.; Chen, T.; Shi, L.; Yang, Y.*; Li, G.*; DNA-oriented shaping of cell features for the detection of rare disseminated tumor cells, Anal. Chem., 2019, 91: 1126-1132.
17. Gao, T.; Chai, W.; Shi, L.; Shi, H.; Sheng, A.; Yang, J.*; Li, G.*; A new colorimetric assay method for the detection of anti-hepatitis c virus antibody with high sensitivity, Analyst, 2019, 144: 6365-6370.
18. Gao, T.; Chen, T.; Feng, C.; He, X.; Mu, C.; Anzai, J. I.; Li, G.*; Design and fabrication of flexible DNA polymer cocoons to encapsulate live cells, Nat Commun, 2019, 10: 2946.
19. Gao, T.; Li, G.* Design, Principle and Application of Self-Assembled Nanobiomaterials in Biology and Medicine-Bio-interfacial DNA self-assemblies for biomedical applications. Elsevier, 2022.（专著/章节）
20. Zhu, X. L.; Gao, T.* Nano-inspired Biosensors for Protein Assay with Clinical Applications, Spectrometry, Elsevier, 2019.（专著/章节）
21. 高涛，杨嘉华，黄琼波，赵松岩，韦天香。一种光热响应DNA修饰的金纳米颗粒制备方法及核酸可视化扩增应用。专利号：202011198874.6。（专利）
22. 高涛。一种基于光亲和共价连接的蛋白质分子弱相互作用捕获磁珠及其制备方法和应用。申请号：PCT/CN2020/116950。（专利）
23. 高涛，郭志刚，何梦元，杨洋，韦天香。一种基于光亲和共价连接的蛋白质分子弱相互作用捕获磁珠的制备方法及其应用。专利号：ZL201911247058.5。（专利）
24. 李根喜，高涛，李金龙，杨大威，谷时雨。一种基于DNA分子级联信号放大的膜蛋白分析方法。专利号：ZL 2016102324390.7。（专利）
二、生物电化学与生物传感器（Bioelectrochemistry & Biosensors）
1. Zhao, J.; Lei, Y.; Wang, P.; Hu, M.; Wang, X.; Wei, T.*; Wang, Z.*; Gao, T.*; Biomineralized electroactive microbe as the whole-cell electrical transducer for electrochemically online monitoring of lactic acid fermentation, Anal. Chem., 2025, 97: 20527-20535.
2. Xie, H.; Wang, L.; Wang, G.; Deng, Y.; Hu, X.; Lin, Y.; Zhang, D.; Gao, T.*; Xie, P.*; Li, G.*; Preparation of living material with metal-organic frameworks and bacteria for the design of electrochemical biosensor to detect exosomes, Small, 2025, 21: e08741.
3. Wang, B. G.; Zhu, J.; Wei, T. X.; Zhang, Y. X.; Li, M. Y.; Zhang, Z. L.; Gao, T.*; Dai, Z. H.*; Interactional analysis of single mitochondrial function directly in living cell reveals the proton circuit decoupling of mitochondria at the preliminary stage of apoptosis, CCS Chemistry, 2025, 7: 205-215.
4. Wu, X.; Zhang, J.; Hu, M.; Wei, W.; Wei, T.*; Gao, T.*; Micro- and nano-scaled microbial electrochemical biosensing, Small Methods, 2025: e01370.
5. Zhang, Y.; Zhu, J.; Zhao, J.; Wang, X.; Wei, T.; Gao, T.*; A single-microbe living bioelectronic sensor for intracellular amperometric analysis, Biosens. Bioelectron., 2024, 265: 116648.
6. Wang, B.; Zhao, J.; Zhang, J.; Wei, T.*; Han, K.*; Gao, T.*; Electrochemical biosensing interfaced with cell-free synthetic biology, TrAC-Trends Anal. Chem., 2024, 176, 117756.
7. Zhu, J.; Wang, B.; Zhang, Y.; Wei, T.; Gao, T.*; Living electrochemical biosensing: Engineered electroactive bacteria for biosensor development and the emerging trends, Biosens. Bioelectron., 2023, 237: 115480.
8. Guo, J.; Li, M.; Long, S.; Zhu, J.; Miao, P.; Wei, T.; Gao, T.*; Bio-inspired electrochemical detection of nitric oxide promoted by coordinating the histamine-iron phthalocyanine catalytic center on microelectrode, Anal. Chem., 2023, 95: 8842-8849.
9. Wang, X.; Gao, T.*; Zhu, J.; Long, S.; Zhao, S.; Yuan, L.*; Wang, Z.*; Fabrication of channeled and three-dimensional electrodes for the integrated capture and detection of invasive circulating tumor cells during hematogenous metastasis, Anal. Chem., 2023, 95: 2496-2503.
10. Shi, L.; Kuang, D.; Ma, X.; Jalalah, M.; Alsareii, S. A.; Gao, T.*; Harraz, F. A.*; Yang, J.; Li, G.*; Peptide assembled in a nano-confined space as a molecular rectifier for the availability of ionic current modulation, Nano Lett., 2022, 22: 1083-1090.
11. Guo, J.; Wei, T.; Huang, Q.; Li, M.; Yang, C.; Mou, J.; Shi, L.; Gao, T.*; Li, G.*; Direct acupuncture of nitric oxide by an electrochemical microsensor with high time-space resolution, Biosens. Bioelectron., 2022, 195: 113667.
12. Wang, L.; Li, H.; Shi, L.; Li, L.; Jia, F.; Gao, T.*; Li, G.*; In situ peptide self-assembly on ionic nanochannel for dynamic monitoring of MMPs in extracellular matrix, Biosens. Bioelectron., 2022, 195: 113671.
13. Shi, L.; Wang, L.; Ma, X.; Jalalah, M.; Alsareii, S. A.; Gao, T.*; Harraz, F. A.*; Li, G.*; Electrochemical trans-channel assay for efficient evaluation of tumor cell invasiveness, ACS Appl Mater Interfaces, 2021, 13: 17268-17275.
14. Shi, L.; Jia, F. J.; Wang, L.; Jalalah, M.; Al-Assiri, M.; Gao, T.*; Harraz, F. A.*; Li, G. X.*; Fabrication of an artificial ionic gate inspired by mercury-resistant bacteria for simple and sensitive detection of mercury ion, Sens. Actuator B-Chem., 2021, 326.
15. Wei, T.; Xu, Q.; Zou, C.; He, Z.; Tang, Y.; Gao, T.; Han, M.*; Dai, Z.*; A boronate-modified renewable nanointerface for ultrasensitive electrochemical assay of cellulase activity, Chin. Chem. Lett., 2021, 32: 1470-1474.
16. Zheng, J.; Gao, T.; Shi, H.; Huang, Y.; Xiang, Y.*; Li, G.*; Electrochemical analysis of 8-hydroxy-2'-deoxyguanosine with enhanced sensitivity based on exonuclease-mediated functional nucleic acid, Talanta, 2019, 199: 324-328.
17. Shi, L.; Mu, C.; Gao, T.; Chai, W.; Sheng, A.; Chen, T.; Yang, J.; Zhu, X.*; Li, G.*; Rhodopsin-like ionic gate fabricated with graphene oxide and isomeric DNA switch for efficient photocontrol of ion transport, J. Am. Chem. Soc., 2019, 141: 8239-8243.
18. Zhao, J. M.; Gao, T.* Genetic Engineering of Microorganisms with Electroactive Genes for the Fabrication of Electrochemical Microbial Biosensors. Methods in Molecular Biology- Synthetic Promoters, Springer Nature, 2024, 2844, 247–260.（专著/章节）
19. Zheng, J.; Fang, X. X.; Wei, T. X.; Gao, T.*. Engineered Living Materials: Applications in Electrochemical Biosensing and Diagnostics. Handbook of Material Engineering in Nanobiomedicine and Diagnostics, Springer Nature, 2024, in Press.（专著/章节）
20. 高涛，郭嘉荣，牟俊慧，杨翠，徐晨韵。一种一氧化氮电化学传感微电极的制备方法及其应用。专利号：ZL202110556466.X。（专利）
【教学培养 / Teaching】
主讲课程：《现代生物学实验技术》、《分子生物学》、《细胞生物学实验》等。
本科生：指导大学生创新创业项目（国家级2项，校级1项），指导本科生发表研究论文4篇（SCI 2篇，中文核心2篇），申请专利2项。
研究生：获国家奖学金6人，校长奖学金2人，江苏省研究生创新计划资助2人，校优秀毕业生3人，校优秀研究生4人，一等学业奖学金多人。
【联系方式 / Contact Information】
通讯地址：江苏省南京市栖霞区文苑路1号，南京师范大学 生命科学学院。邮编：210023。电子邮件: taogao@njnu.edu.cn
