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许凯

南京师范大学生命科学学院微生物与生物技术研究所教授,博士生导师。获得国家海外高层次人才引进计划、江苏省特聘教授、江苏省杰出青年基金资助。主要从事植物病毒学、病毒与寄主互作机制的研究,近年来在PLoS Biology, PNAS, Molecular Plant, PLOS Pathogens, Journal of Virology, Current Opinion in Virology等杂志上发表论文20多篇,主要研究兴趣集中在膜脂分子及膜脂运输途径参与植物正义RNA病毒复制的过程和机制。

膜脂分子是真核细胞内重要的生物大分子,参与组成细胞器并参与细胞内许多重要的代谢途径。膜脂也是正义RNA病毒复制所必须的关键寄主因子。本课题组利用导致我国大豆花叶病的主要病原之一大豆花叶病毒(Soybean mosaic virus, SMV)以及烟草坏死病毒A中国大豆分离物(Tobacco necrosis virus-Ac, TNV-Ac)为实验对象,通过对膜脂分子合成和运输途径中关键基因进行分析,及在体外建立膜脂与蛋白互作研究体系,揭示膜脂及关键脂转运蛋白参与植物病毒基因组复制、粒子装配及病毒传播的机理,以找到可以控制这一类大豆病毒病害的靶标途径。

本课题组充分利用分子生物学、生物化学、细胞生物学、遗传学、蛋白组和脂质组的技术手段,通过对植物病毒致病机制及植物的抗病机理的研究,对实验室成员进行相关科学知识和实验能力的全面培养与训练,欢迎具有生命科学相关学科背景的本科生、研究生和博士加入,进行本科毕业设计、研究生学习或博士后工作。

 

教育背景与工作经历:

2017.1至今南京师范大学生命科学学院教授;

2014.7– 2016.9美国肯塔基大学植物病理系博士后研究员;

2007.8– 2014.6美国肯塔基大学植物病理系博士研究助理,获博士学位;

2005.7– 2007.3中国农业大学农业生物技术国家重点实验室科研助理;

2001.9– 2005.6中国农业大学生物学院生物科学理科基地班,获理学学士学位。

 

论文著作:

  1. Feng Z, Xu K*(*共同通讯作者), Kovalev N, Nagy PD*. 2019,Recruitment of Vps34 PI3K and enrichment of PI3P phosphoinositide in the viral replication compartment is crucial for replication of a positive-strand RNA virus. PLoS Pathogens, 15(1): e1007530.
  2. Yang M, Li Z, Zhang K, Zhang X, Zhang Y, Wang X, Han C, Yu J, Xu K*(*共同通讯作者), Li D*. 2018,Barley stripe mosaic virus γb interacts with glycolate oxidase and inhibits peroxisomal ROS production to facilitate virus infection. Molecular Plant,11(2):338-341.
  3. Xu K, Nagy PD.2017, Sterol-binding by the tombusviral replication proteins is essential for replication in yeast and plants. Journal of Virology,91 (7), e01984-16.
  4. Xu K, Nagy PD.2016, Enrichment of phosphatidylethanolamine in viral replication organelles via co-opting the Rab5 endosomes by a positive-strand RNA virus. PLoS Biology, 14(10): e2000128.
  5. Nawaz-Ul-Rehman MS#, Prasanth KR#, Xu K#(#:共同第一作者), Sasvari Z#, Kovalev N, de Castro Martin IF, Barajas D, Risco C, Nagy PD. 2016, Viral replication protein inhibits cellular cofilin actin depolymerization factor to regulate the actin network and promote viral replicase assembly. PLoS Pathogens,12(2):e1005440.
  6. Xu K, Nagy PD. 2015, RNA virus replication depends on enrichment of phosphatidylethanolamine at replication sites in subcellular membranes. Proceedings of the National Academy of SciencesUSA, 112(14): E1782-E1791.
  7. Xu K, Nagy PD. 2014, Expanding use of multi-origin subcellular membranes by positive-strand RNA viruses during replication. Current Opinion in Virology, 9: 119-126.
  8. Xu K, Lin JY, Nagy PD. 2014, The Hop-like stress induced protein 1 co-chaperone is a novel cell-intrinsic restriction factor for mitochondrial tombusvirus replication. Journal of Virology, 88(16):9361-9378.
  9. Xu K, Huang TS, Nagy PD. 2012, Authentic in vitro replication of two tombusviruses in isolated mitochondrial and endoplasmic reticulum membranes. Journal of Virology, 86(23):12779-12794.
  10. Xu K, Nagy PD. 2010, Dissecting virus-plant interactions through proteomics approaches. Current Proteomics, 7(4): 316-327.
  11. Xu K, Yoshida R. 2010, Statistical analysis on detecting recombination sites in DNA-β satellites associated with old world geminiviruses. Frontiers in Psychiatry, 1: 138. b
  12. Inaba JI, Xu K, Kovalev N, Ramanathan H, Roy CR, Lindenbach BD, Nagy PD.2019,Screening Legionella effectors for antiviral effects reveals Rab1 GTPase as a proviral factor coopted for tombusvirus replication.Proceedings of the National Academy of SciencesUSA, doi: 10.1073/pnas.1911108116.
  13. Wang X, Cao X, Liu M, Zhang R, Zhang X, Gao Z, Zhao X, Xu K, Li D, Zhang Y. 2018, Hsc70-2 is required for Beet black scorch virus infection through interaction with replication and capsid proteins.Scientific Reports,14;8(1):4526.
  14. Zhang X, Dong K, Xu K, Zhang K, Jin X, Yang M, Zhang Y, Wang X, Han C, Yu J, Li D. 2018, Barley stripe mosaic virus infection requires PKA-mediated phosphorylation of γb for suppression of both RNA silencing and the host cell death response.New Phytologistdoi: 10.1111/nph.15065.
  15. Sasvari Z, Kovalev N, Gonzalez PA, Xu K, Nagy PD. 2018, Assembly-hub function of ER-localized SNARE proteins in biogenesis of tombusvirus replication compartment. PLoS Pathogens, 14(5):e1007028
  16. Nagy PD, Pogany J, Xu K. 2016. Cell-free and cell-based approaches to explore the roles of host membranes and lipids in the formation of viral replication compartment induced by Tombusviruses. Viruses, 8(3):68
  17. Barajas D, Xu K, Sharma M, Wu CY, Nagy PD. 2014, Tombusviruses upregulate phospholipid biosynthesis via interaction between p33 replication protein and yeast lipid sensor proteins during virus replication in yeast. Virology, 471:72-80.
  18. Barajas D, Xu K, de Castro Martín IF, Sasvari Z, Brandizzi F, Risco C, Nagy PD. 2014, Co-opted oxysterol-binding ORP and VAP proteins channel sterols to RNA virus replication sites via membrane contact sites. PLoS Pathogens, 10(10):e1004388.
  19. Shah Nawaz-ul-Rehman M, Martinez-Ochoa N, Pascal H, Sasvari Z, Herbst C,Xu K, Baker J, Sharma M, Herbst A, Nagy PD. 2012, Proteome-wide overexpression of host proteins for identification of factors affecting tombusvirus RNA replication: an inhibitory role of protein kinase C. Journal of Virology, 86(17): 9384-9395.
  20. Pathak KB, Pogany J,Xu K, White KA, Nagy PD. 2012, Defining the roles of cis-acting RNA elements in tombusvirusreplicase assembly in vitro. Journal of Virology, 86(1):156-171.
  21. Li Z, Pogany J, Panavas T, Xu K, Esposito AM, Kinzy TG, Nagy PD. 2009, Translation elongation factor 1A is a component of the tombusvirusreplicase complex and affects the stability of the p33 replication co-factor. Virology, 385(1):245-260.
  22. Niu S, Wang B, Guo X, Yu J, Wang X, Xu K, Zhai Y, Wang J, Liu Z., 2009, Identification of two RNA silencing suppressors from banana bunchy top virus. Archives in Virology, 154(11):1775-1783.

 

承担科研项目:

  1. 国家自然科学基金面上项目 (2018 - 2021)
  2. 江苏省自然科学基金杰出青年基金项目 (2018-2021)

 

学术会员:

美国病毒学协会会员 (2010至今)

 

联系方式:

邮件:xukai@njnu.edu.cn

通讯地址:江苏省南京市文苑路1号,南京师范大学生命科学院,邮编:210023