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王文

 

王文  教授、博士生导师

工作单位:南京师范大学生命科学学院 动物遗传资源研究所

通讯地址:江苏省南京市文苑路1号,邮编:210023

电话/传真:025-85891955

E-mail: wenwang@njnu.edu.cn; njnuwang@263.net

基本情况:

女,1957年4月出生,新疆石河子市人。研究生学历,博士学位,教授、博士生导师。江苏省水生甲壳动物病害重点实验室主任。1980年南京师范学院生物系毕业留校工作至今。1989年获南京师范大学生物系理学学士学位,2006获南京大学生命科学学院理学博士学位。1995年任细胞生物学专业硕士生导师,1998年任水产养殖专业硕士生导师,2004年6月任动物学博士生导师,2006年任水生生物学博士生导师2007.12-2008.12 美国哥伦比亚大学微生物学系高级访问学者一年。

主要研究方向:

  1.水生动物疾病学

  2.病原微生物学

  3.水生动物疫病诊断和防治技术

学术兼职:

  1.亚洲支原体学会常务理事

  2.国际螺原体分类标准起草委员会委员

  3.中华医学会支原体分会

  4.南京师范大学学报(工程技术版)编委

  5.江苏省动物学会理事

 

获奖与荣誉称号:

  1. 1994年校首届优秀中青年骨干教师
  2. 1998年江苏省巾帼建功先进个人
  3. 2009年所负责的研究团队荣获江苏省“巾帼文明岗”称号(团队负责人)
  4. 2013年获江苏省五一劳动奖章
  5. 2014年获2013年度江苏省科学技术一等奖(排名第一)
  6. 2014年获2013年度国家教育部自然科学二等奖(排名第一)
  7. 2014年获江苏省渔业巾帼科技能手
  8. 2014年获江苏省科协动物学会首席专家
  9. 2014年获全国优秀科技工作者称号

 

主要科研及成果:

自1998年开展中华绒螯蟹(俗称河蟹)“颤抖病”的研究以来,先后承担与其相关的国家科技部星火计划1项,国家自然科学基金面上项目5项,6项省级项目,完成了从病原发现、病原分离和鉴定、病原的命名、病原基因组学及蛋白组学以及宿主免疫分子机制等一系列研究。发表论文73篇(其中SCI期刊57篇,29篇为同领域前10%期刊),并建立了水产螺原体的快速检测和防控技术,获得国家发明专利5项,相关成果和技术已在江苏省南京、宝应、高邮、兴化、句容等地区进行推广应用,成效显著,建立了一个了从基础研究到核心技术再到应用的一个系统的研究体系,取得了一系列原创性成果。以上科研成果分别获2013年度江苏省科学技术一等奖和2013年度国家教育部自然科学奖二等奖。

 

学术交流与合作:

  1. 以分会主席和特邀发言人的身份参加境外国际会议(日本、英国、韩国、法国、意大利等)7次,国内一级(中国微生物学会)大会报告1次。
  2. 目前与国际4个实验室建立了合作关系:

Gasparich 教授实验室:国际知名螺原体分子生物学研究专家,国际支原体学会分类委员会委员、美国陶森大学(Towson University) Fisher College of Science and Mathematics副主任、教授。

Shields 教授实验室:前国际甲壳动物学会主席、水生甲壳动物病害专家、美国弗吉尼亚海洋科学院(Virginia Institute of Marine Science, USA)国家海洋科学学院环境与水生动物系蟹病研究室主任Jeffrey D. Shields博士。

Miyata教授实验室 日本大阪市立大学生物学系宫田真人(Makoto Miyata)教授,国际著名支原体研究专家,在鱼类致病菌——滑动支原体的运动机制研究方面很有造诣,论文发表在PNAS,JBC等国际知名期刊。目前已派博士生前往Miyata教授实验室合作开展河蟹螺原体细胞骨架及运动机制等发明的研究。

Shigeyuki Kakizawa (Principal Investigator) National Institute of Advanced Industrial Science and Technology (AIST) in Japan(日本产业技术综合研究所),与本实验室开展有关虾蟹螺原体基因组学方面的合作和研究。

 

 

  1. Amaro F, Wang W, Gilbert JA, Roger Anderson O, Shuman HA. Diverse protist grazers select for virulence-related traits in Legionella. ISME J. 2015 1-12. (IF=9.267).
  2. Xiu Y, Wu T, Meng X, Meng Q*, Wang W*. Identification and isolation of a spiroplasma pathogen from diseased oriental river prawn, Macrobrachium nipponense, in China: A new freshwater crustacean host. Aquaculture. 2015, 437: 270-274.
  3. Huang Y1, Ma F1, Wang W*, Ren Q*. Identification and molecular characterization of a peritrophin-like gene, involved in the antibacterial response in Chinese mitten crab, Eriocheir sinensis. Dev Comp Immunol. 2015, 50:129-138.
  4. Huang X, Huang Y, Shi Y, Ren Q*, Wang W* Function of a novel C-type lectin with two CRD domains from Macrobrachium rosenbergii in innate immunity. Dev Comp Immunol. 2014, 46 (2):255-266.
  5. Huang Y, Chen H, Wang Z, Wang W*, Ren, Q* Novel myeloid differentiation factor 88, EsMyD88, exhibits EsTube-binding activity in Chinese mitten crab Eriocheir sinensis Dev Comp Immunol. 2014, 47:298-308.
  6. Huang Y, Huang X, Hou L, An L, Hui K, Ren Q*, Wang W* Molecular cloning and characterization of three novel Hemocyanins from Chinese mitten crab,Eriocheir sinensis  Aquaculture 2014, 434: 385-396.
  7. Huang Y, An L, Hui K, Ren Q*, Wang W* An LDLa domain-containing C-type lectin is involved in the innate immunity of Eriocheir sinensis Dev Comp Immunol 42: 333-344, 2014.
  8. Huang Y, Huang X, Wang Z, Tan J, Hui K, Wang W*, Ren Q* Function of two novel single-CRD containing C-type lectins in innate immunity from Eriocheir sinensis Fish Shellfish Immunol 37 :313-321, 2014.
  9. Huang Y, Tan J, Wang Z, Yin S, Huang X, Wang W*, Ren Q* Cloning and characterization of two different L-type lectin genes from the Chinese mitten crab Eriocheir sinensis Dev Comp Immunol 46: 255-266, 2014. (SCI)
  10. 吴霆,丁正峰,朱春艳,薛中仪,顾伟,孟庆国,王文*,异育银鲫鳃出血病流行病学调查和研究,水产科学, 33(5):283-287, 2014.
  11. 吴霆,丁正峰,朱春艳,薛中仪,顾伟,孟庆国,王文*,江苏省宝应县异育银鲫鳃疱疹病毒病流行病学调查和研究,科学养鱼,7: 52-53, 2014.
  12. Meng Q, Hou L, Zhao Y, Huang X, Huang Y, Xia S, Gu Wei, Wang W*. iTRAQ-based proteomic study of the effects of Spiroplasma eriocheiris on Chinese mitten crab Eriocheir sinensis hemocytes Fish Shellfish Immunol, 40:182-189, 2014.
  13. Gu W, Chen J, Hou L, Huang Y, Xia S, Meng Q, Wang W*. The Superoxide Dismutase from Red Claw Crayfish, Cherax quadricarinatus Molecular Cloning and Characterization Analysis. See comment in PubMed Commons belowZoolog Sci. 31(11):725-734. 2014.
  14. Gu W, Yao W, Zhao Y, Pei S, Jiang C, Meng Q, Wang W*. Establishment of spiroplasma-infected hemocytes as an in vitro laboratory culture model of Chinese mitten crab Eriocheir sinensis Vet Microbiol. 171(1-2):215-220, 2014.
  15. Xiu Y, Feng J, Lu W, Liu D, Wu T, Zhu H, Liu P, Li W, Ren Q, Gu W, Meng Q, Wang W*. Identification of a novel cognate cytosolic Hsp70 gene (MnHsc70-2) from oriental river prawn Macrobrachium nipponense and comparison of its expressions with the first cognate Hsc70 (MnHsc70-1) under different stresses. Cell Stress Chaperones. 19(6): 949-961,2014
  16. Xiu Y, Wu T, Liu P, Huang Y, Ren Q, Gu W, Meng Q, Wang W* Molecular cloning and characterization of the lipopolysaccharide and b-1,3-glucan binding protein from oriental river prawn, Macrobrachium nipponenseMol Biol Rep  41:3935–3944, 2014.
  17. Ding Z, Tang J, Xue H, Li J, Ren Q, Gu W, Meng Q*, Wang W*. Quantitative detection and proliferation dynamics of a novel Spiroplasma eriocheiris pathogen in the freshwater crayfish, Procambarus clarkii. J Invertebr Pathol, 115:51-54, 2014.
  18. Wu T, Ding Z, Ren M, An L, Xiao Z, Liu P, Gu W, Meng Q*, Wang W*. The histo- and ultra-pathological studies on a fatal disease of Prussian carp (Carassius gibelio) in mainland China associated with cyprinid herpesvirus 2 (CyHV-2).  Aquaculture 412-413: 8-13, 2013.
  19. Huang Y, Li YR, An L, Hui KM, Ren Q*, Wang W*. Cloning and characterization of a clip domain serine protease and its homolog (masquerade) from Eriocheir sinensis. Fish Shellfish Immunol. 35(4):1155-1162. 2013.
  20. Zhu H, Du J, Hui K, Liu P, Chen J, Xiu Y, Yao W, Wu T, Ren Q, Meng Q, Gu W, Wang W*. Diversity of lectins in Macrobrachium rosenbergii and their expression patterns under spiroplasma MR-1008 stimulation. Fish Shellfish Immunol. 2013. 35(2): 300-309, 2013.
  21. Hui K, Hao F, Li W, Zhang Z, Zhang CY, Wang W*, Ren Q*. Cloning and identification of four Mu-type glutathione S-transferases from the giant freshwater prawn Macrobrachium rosenbergii. Fish Shellfish Immunol. 35(2):546-552, 2013.
  22. Ou J, Li Y, Ding Z, Xiu Y, Wu T, Du J, Li W, Zhu H, Ren Q, Gu W, Wang W* Transcriptome-wide identification and characterization of the Procambarus clarkii microRNAs potentially related to immunity against Spiroplasma eriocheiris infection. Fish Shellfish Immunol. 35(2): 607-617, 2013.
  23. Meng Q, Chen J, Xu C, Huang Y, Wang Y, Wang T , Zhai X, Gu W, Wang W* The characterization, expression and activity 1 analysis of superoxide 2 dismutases (SODs) from Procambarus clarkii Aquaculture, 406-407:131-140, 2013.
  24. Ding Z, Yao W, Du J, Ren Q, Li W, Wu T, Xiu Y, Meng Q, Gu W, Xue H, Tang J, Wang W*, Histopathological characterization and in situ hybridization of a novel spiroplasma pathogen in the freshwater crayfish Procambarus clarkii Aquaculture 380-383:106-113, 2013.
  25. Ren Q*, Zhong X, Yin S, Hao F, Hui K, Zhang Z, Zhang C, Yu X, Wang W*, The first Toll receptor from the triangle-shell pearl mussel Hyriopsis cumingii, Fish Shellfish Immunol, 34(5):1287-1293. 2013.
  26. Xiu Y., Wu T., Du J., Yao W., Li W., Ding Z., Meng Q., Ren Q., Gu W., Wang W*. Molecular characterization and expression analysis 1 of extracellular copper/zinc superoxide dismutase (ecCuZnSOD) from oriental river prawn, Macrobrachium nipponense Aquaculture. 380-383:23-28, 2013.
  27. Du J, Zhu H, Liu P, Chen J, Xiu Y, Yao W, Wu T, Ren Q, Meng Q, Gu W, Wang W*. Immune responses and gene expression in hepatopancreas from Macrobrachium rosenbergii challenged by a novel pathogen spiroplasma MR-1008. Fish Shellfish Immunol.,34(1):315-323, 2013.
  28. 孟庆国, 黄艳青, 靳明建, 顾伟, 王文*,中华绒螯蟹螺原体对中华绒螯蟹和RAW264.7细胞免疫反应研究, 南京师大学报(自然科学版),35(2):1-6. 2012.
  29. 孟庆国, 陈静, 黄艳青, 靳明建, 顾伟, 王文*,红螯螯虾冷休克蛋白Y-box编码基因的克隆及表达分析, 淡水渔业 2012,42(6):1-7
  30. Du J., Zhu H., Ren Q., Liu P., Chen J., Xiu Y., Yao W., Meng Q., Gu W., Wang W*. Flow cytometry studies on the Macrobrachium rosenbergii hemocytes sub-populations and immune responses to novel pathogen spiroplasma MR-1008. Fish Shellfish Immunol. 33(4):795-800, 2012.
  31. Ren Q*, Qi YL, Hui KM, Zhang Z, Zhang CY, Wang W.* Four invertebrate-type lysozyme genes from triangle-shell pearl mussel (Hyriopsis cumingii). Fish Shellfish Immunol 33(4):909-915, 2012.
  32. Ren Q*, Zhang Z, Li XC, Jie-Du, Hui KM, Zhang CY, Wang W*. Three different anti-lipopolysaccharide factors identified from giant freshwater prawn, Macrobrachium rosenbergii Fish Shellfish Immunol. 33(4):766-77, 2012.
  33. Liang T., Ji H., Du J., Ou J., Li W., Wu T., Meng Q., Gu W., Wang W*.  Primary culture of hemocytes from Eriocheir sinensis and their immune effects to the novel crustacean pathogen Spiroplasma eriocheiris  Molecular Biology Report 39(10):9747-54, 2012.
  34. Ding Z., Du J., Ou J., Li W., Wu T., Xiu Y., Meng Q., Wu W., Xue H., Tang J., Wang W*. Classification of circulating hemocytes from the red swamp crayfish Procambarus clarkii and their susceptibility to the novel pathogen Spiroplasma eriocheiris in vitro Aquaculture 356-357:371-380, 2012.
  35. Ren Q*, Li M, Du J, Zhang CY, Wang W*.  Immune response of four dual-CRD C-type lectins to microbial challenges in giant freshwater prawn Macrobrachium rosenbergii Fish Shellfish Immunol..33(2):155-167, 2012.
  36. Ou J, Meng Q, Li Y, Xiu Y, Du J, Gu W, Wu T, Li W, Ding Z, Wang W*. Identification and comparative analysis of the Eriocheir sinensis microRNA transcriptome response to Spiroplasma infection using a deep sequencing approach. Fish Shellfish Immunol 32:345-352, 2012.
  37. Du J, Ou J, Li W, Ding Z, Wu T, Meng Q, Gu W, Wang W*. Primary hemocytes culture of the freshwater prawn Macrobrachium rosenbergii and their susceptibility to the novel pathogen spiroplasma strain MR-1008. Aquaculture 330-333: 21-28, 2012.
  38. Liang T, Wu T, Du J, Ji H, Li Y, Gu W, Wang W* Characterization of a tailless white spot syndrome virus from diseased Penaeus vannamei and Procambarus clarkii in China Afr J Biotechnol. 10:13936-13942, 2011.
  39. Anderson OR, Wang W, Faucher SP, Bi Keran, Shuman HA. A New Heterolobosean Amoeba Solumitrus palustris n. g., n. sp. Isolated from Freshwater Marsh Soil. J Eukaryot Microbiol., 58: 60–67, 2011.
  40. Meng Q, Du Jie, Yao W, Xiu Y, Li Y, Gu W, Wang W* An extracellular copper/zinc superoxide dismutase (ecCuZnSOD) from Chinese mitten crab, Eriocheir sinensis and its relationship with Spiroplasma eriocheiris  Aquaculture 320:56-61, 2011.
  41. Liang T, Li X, Du Jie, Yao W, Sun G, Dong X, Liu Z, Ou J, Meng Q, Gu W, Wang W* Identification and isolation of a spiroplasma pathogen from diseased freshwater prawns, Macrobrachium rosenbergii, in China: A new freshwater crustacean host. Aquaculture  318: 1-6, 2011.
  42. Wang W.*, Gu W. Gasparich GE. Bi K., Ou J., Meng Q., Liang T.,  Feng Q., Zhang J., Zhang Y. Spiroplasma eriocheiris sp. nov., a novel species associated with mortalities in Eriocheir sinensis, Chinese mitten crab. Int J Syst Evol Microbiol, 61:2745-2752, 2011.
  43. 顾伟,张秋萍,张莉,李菲,王文*,陈辉,邹勇,吴霆,华伯仙,江苏省中华绒螯蟹螺原体疫病的检测和普查江苏农业科学,1:293-295, 2011
  44. Feng Q., Wu G., Liang T., Ji H., Jiang X., Gu W., Wang W.* Pharmacokinetics of oxytetracycline in hemolymph from the Chinese mitten crab, Eriocheir sinensis J Vet Pharmacol Therap, 34:51-57, 2011. (SCI)  
  45. Wang W* Bacterial diseases of crabs: A review J Invertebr Pathol, 106:18-26, 2011.
  46. Meng Q., Li W.,  Liang T., Jiang X., Gu W., Wang W.* Identification of adhesin-like protein ALP41 from Spiroplasma eriocheiris and induction immune response of Eriocheir sinensis Fish Shellfish Immunol, 29:587-593, 2010.
  47. Meng Q., Ou J., Ji H., Jiang X., Gu W., Wang W.* Identification and characterization of spiralin-like protein SLP25 from Spiroplasma eriocheiris Vet Microbiol, 4:473-477, 2010.
  48. Feng Q., Gao T., Ji H., Jiang X., Liang T., Gu W., Wu G., Gao G., Wang W.* Kinetic analysis of oxytetracycline residues in Chinese mitten crab, Eriocheir sinensis, muscle following intramuscular administration.  J Fish Dis, 33: 639-647, 2010.
  49. Liang T., Ji H., Lian L., Wu T., Gu W., Wang W.* A rapid assay for simultaneous detection of Spiroplasma eriocheiris and white spot syndrome virus in Procambarus clarkii by multiplex PCR Lett Appl Microbiol, 51:532-538, 2010.
  50. Meng Q., Ji H., Bi K., Jiang X., Gu W., Wang W.* Spiralin-like protein SLP31 from Spiroplasma eriocheiris as a potential antigen for immunodiagnostics of tremor disease in Chinese mitten crab Eriocheir sinensis Folia Microbiol, 55:245-250, 2010.
  51. 吴霆, 华伯仙, 顾伟,王文*, 中华绒螯蟹颤抖病诊断和防治技术, 中国水产, (8): 59-61,2010.
  52. Liang T., Feng Q., Wu T., Gu W., Wang W.* Use of oxytetracycline for the treatment of tremor disease in the Chinese mitten crab Eriocheir sinensis Dis Aquat Organ, 84:243-250, 2009.
  53. Wang J., Huang H., Feng Q., Liang T., Bi K., Gu W., Wang W.* Enzyme-lined immunosorbent assay for the detection of pathogenic spiroplasma in commercially exploited crustaceans from China  Aquaculture, 92:166-171, 2009.
  54. 吴霆, 王文*, 河蟹颤抖病综合预防与控制关键技术, 科学养鱼, 11:50-51, 2009.
  55. Bi K., Huang H., Gu W., Wang J., Wang W.* Phylogenetic analysis of Spiroplasmas from three freshwater crustaceans (Eriocheir sinensis, Procambarus clarkia and Penaeus vannamei) in China J Invertebr Pathol, 99:57-65, 2008.
  56. Bi K., Gu W., Wang W.* Sensitive and rapid detection of freshwater crustacean Spiroplasmas by ISRs-sequence-targeted species-specific primers Eur Food Res Technol, 227:1733-1737, 2008.
  57. 吴霆, 顾伟, 王文*, 中华绒螯蟹颤抖病药物治疗的初步研究, 水产科学, 27:325-329, 2008.
  58. 吴霆,可然,王文* 套式PCR在中华绒螯蟹颤抖病病原螺原体检测中的应用, 水产科学, 26:551-553, 2007.
  59. Ding Z., Bi K, Wu T., Gu W., Wang W. *, Chen J., A simple PCR method for the detection of pathogenic spiroplasmas in crustaceans and environmental samples, Aquaculture 265: 49–54, 2007.
  60. Wang W. *, Chen J., Ultrastructural study on a novel microsporidian, Endoreticulatus eriocheir sp. nov (Microsporidia, Encephalitozoonidae), parasite of Chinese mitten crab, Eriocheir sinensis (Crustacea, Decapoda), J Invertebr Pathol. 94:77–83, 2007.
  61. Wang W*., Gu W., Ding Z., Ren Y., Chen J., Hou Y. A novel Spiroplasma Pathogen Causing Systemic Infection in the Crayfish Procambarus Clarkii (Crustacea:Decapod), in China. FEMS Microbiol Lett. 249(1): 131-137, 2005.
  62. Wang W*., Wen B., Gasparich G.E., Zhu N., Rong L., Chen J., Xu Z. A spiroplasma associated with tremor disease in the Chinese mitten crab (Eriocheir sinensis). Microbiology.150: 3035-3040, 2004.
  63. Wang W*., Chen J., Du  K., Xu Z. Morphology of spiroplasmas in the Chinese mitten crab Eriocheir sinensis associated with tremor disease. Res Microbiol. 155(8): 630-635, 2004.
  64. Wang W*., Rong L., Gu W., Du K.,  Chen J. Study on experimental infections of TDA from the Chinese mitten crab in crayfish, mice and embryonated chickens. Res Microbiol. 154(10): 677-680, 2003.
  65. Wang W*., Gu Z. Rickettsia-like Organism Associated with Tremor Disease and Mortality of the Chinese Mitten Crab, Eriocheir sinensis. Dis Aquat Organ. 48(2): 149-153,2002.
  66. Wang W*., Zhu N., Gu Z., Du K., Xu Z. Study on the Transmission of Tremor Disease (TD) in the Chinese Mitten Crab, Eriocheir sinensis (Crustacea: Decapoda). J Invertebr Pathol. 81(3): 202-204, 2002. (SCI)
  67. 朱宁宁,王文*,李正荣,顾志峰. 患“颤抖病”中华绒螯蟹血细胞中类立克次体寄生的超微结构观察. 电子显微学报. 1: 21-24, 2002.
  68. 王文*,朱宁宁,李正荣,顾志峰, 徐在宽. 类立克次体侵染中华绒螯蟹神经组织的光镜和电镜观察. 动物学研究.6: 467-471, 2001.
  69. 顾志峰,王文*,朱宁宁,吴庆龙, 李文朝, 陈亚芬. 河蟹“颤抖病”病原初步研究. 水产养殖. 1: 17-19, 2001.
  70. 王文*, 顾志峰, 朱宁宁, 李正荣, 杜开和, 徐在宽.患颤抖病中华绒螯蟹体内类立克次体侵染的光镜和电镜观察. 中国水产科学. 4: 32-35, 2001.
  71. 王文*, 朱宁宁, 顾志峰, 李正荣, 徐在宽.应当引起重视的一种水生动物微生物病原体──类立克次体(RLOs). 中国水产. 5: 39-39, 2001.
  72. 顾志峰,王文*,朱宁宁,吴庆龙, 李文朝, 陈亚芬. 患“颤抖病”中华绒螯蟹体内类立克次体生物的寄生. 湖泊科学. 3: 284-290, 2000.
  73. 顾志峰,王文*,朱宁宁,吴庆龙, 李文朝, 陈亚芬.中华绒螯蟹“颤抖病”病原、病理学初步研究. 湖泊科学. 4: 367-372, 2000.