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黄鹰

黄鹰, 博士,南京师范大学教授,博士生导师。1965年出生,1986年毕业于华东理工大学生物化学工程系生物化学工程专业,获工程学学士学位。1986年至1992年在中科院上海生物工程实验基地从事基因工程研究。1997年毕业于美国德州大学卫生研究中心圣安东尼奥分校生物化学系生物化学专业,获博士学位。1997年至2006年在美国国立卫生研究院(NIH) 尤尼斯·肯尼迪·史瑞沃国家儿童健康与人类发展研究所(NICHD)工作,历任Visiting fellow与Research fellow。2006年至2014年7月南京师范大学生命科学学院特聘教授,2014年8月至今,南京师范大学生命科学学院教授。先后获得5项国家自然科学基金面上项目。以第一作者与通讯作者发国际高水平SCI论文20多篇。

研究内容:

  1. 裂殖酵母中帕金森相关基因DJ-1功能的研究
  2. 线粒体RNA合成与蛋白质翻译机制的研究
  3. 酵母中自噬机制的研究

 

欢迎报考微生物学专业或生物技术专业硕士学位或博士学位。

联系电话:办公室:(025)85891263, 手机:13951994845

Email: y_shang_hai@126.com

 

教学

研究生选修课程:微生物遗传学 3 学分54个学时

本科生选修课程:普通免疫学 2 学分36个学时

 

基金项目:

目前主持的科研基金项目:

1.国家自然科学基金(2015.1-2018.12),裂殖酵母线粒体tRNA5'加工酶RNaseP的鉴定与功能研究,80万,项目批准号:31470778

2.国家自然科学基金(2012.1-20015.12),线粒体tRNA前体加工与细胞周期调控之间偶联机制的研究,项目批准号:31170065

 

已完成的科研基金项目:

3.与前体腺癌易感基因相关的、编码裂殖酵母tRNA3'末端加工酶tRNase Z基因功能的研究; 项目批准号:31070703,2011.1-2013.12

4.与前列腺癌相关基因HsaTRZ2/ELAC2同源、编码tRNA 3'末端加工酶的粟酒酵母基因trz1+ 的研究;项目批准号:30670446,2007.1-2009.12

5.与前列腺癌相关基因HsaTRZ2/ELAC2同源的粟酒酵母线粒体蛋白trz2+ 的研究;  项目批准号:30771178,2008.1-2010.12

 

研究论文(*通讯作者)

  1. Zhao, Q., Su, Y., Wang, Z., Chen, C, Wu, T and Huang, Y.* Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe. BMC Evol. Biol., 14: 86 (2014)
  2. Zhang, X., Zhao, Q. and Huang, Y.* Partitioning of the nuclear and mitochondrial tRNA 3′-end processing activities between two different proteins in Schizosaccharomyces pombe. J. Biol. Chem., 288, 27415-27422 (2013)
  3. Sun, L., Yang, X., Chen, F., Li, R., Li, X., Liu, Z., Gu, Y., Gong, X., Liu, Z., Wei, H., Huang, Y. and Yuan, S.* Paralogous Ribosomal Proteins L32-1 and L32-2 in Fission Yeast May Function Distinctively in Cellular Proliferation and Quiescence by Changing the Ratio of Rpl32 Paralogs. PLoS ONE 8(4): e60689doi:10.1371/journal.pone.0060689  (2013)
  4. Li, R., Li, X., Sun, L., Chen, F., Liu, Z., Gu, Y., Gong, X., Liu, Z., Wei, H., Huang, Y. and Yuan, S.* Reduction of Ribosome Level Triggers Flocculation of Fission Yeast Cells. Eukaryot. Cell, 12, 450-459 (2013)
  5. Wang, Z., Zheng, J., Peng, J., Zhang, X., Liu, J. and Huang, Y.* Identification and sequence analysis of metazoan tRNA 3'-end processing enzymes tRNase Zs. PLoS ONE;7(9):e44264. doi: 10.1371/journal.pone.0044264 (2012)
  6. Chen, P, Gao, R, Chen, S, Pu, L, Li, P., Huang, Y., and Lu, L.* A Pericentrin-Related Protein Homolog in Aspergillus nidulans plays Important Roles in Nucleus Positioning and Cell Polarity by affecting microtubule organization. Eukaryot. Cell, 11, 1520-1530 (2012)
  7.  Fan, L., Wang, Z, Liu, J., Guo, W., Yan, J. and Huang, Y.* A survey of green plant tRNA 3'-end processing enzyme tRNase Zs, homologs of the candidate prostate cancer susceptibility protein ELAC2. BMC Evol. Biol., 11: 219 (2011)
  8. Gan, X., Yang, J., Li, J., Yu, H., Dai, H. and Huang, Y.* The fission yeast Schizosaccharomyces pombe has two distinct tRNase ZLs encoded by two different genes and differentially targeted to the nucleus and mitochondria. Biochem J. 435, 103-111 (2011)
  9. Wu, G., Sun, Y, Qu W, Huang, Y., Lu L, Li, L and Shao, W.* Application of GFAT as a novel selection marker to mediate gene expression. PLoS ONE 6(2): e17082. doi:10.1371/journal.pone.0017082 (2011)
  10. Zhao, W., Yu, H., Li, S. and Huang, Y.* Identification and analysis of candidate fungal tRNA 3'-end processing endonucleases tRNase Zs, homologs of the putative prostate cancer susceptibility protein ELAC2. BMC Evol. Biol., 10:272 (2010)
  11. Zhao, Z., Su, W., Yuan S. and Huang, Y.* Functional conservation of tRNase ZL among Saccharomyces cerevisiae, Schizosaccharomyces pombe and humans. Biochem. J., 422, 483-492 (2009)
  12. Yee, N. S., Gong, W., Huang, Y., Lorent, K., Dolan, A. C., Maraia, R. J. and Pack, M. Mutation of zebrafish rpc2 reveals exquisite dependence on RNA polymerase III for the development of the digestive system. PLoS Biology, 5(11), e312 doi:10.1371/journal.pbio.0050312 (2007)
  13. Huang, Y., Bayfield, M., Intine, R. and Maraia, R. J. Separate RNA-binding surfaces on the multifunctional La protein mediate distinguishable activities in tRNA maturation. Nat. Struct. Mol. Biol., 13,611-618 (2006)
  14. Huang, Y., Intine, R., Mozlin, A., Hasson, S. and Maraia, R. J. Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3' oligo-U length and La-dependent tRNA processing in fission yeast. Mol. Cell. Biol., 25, 621-636 (2005)
  15. Huang, Y., Hamada, M. and Maraia, R. J. RNA polymerase III from the fission yeast, S. pombe. In S. Adhya and S. Garges, (eds), Methods Enzymol., 370, Academic Press, Inc., San Diego, CA, pp.165-173 (2003)
  16. Huang, Y., McGillicuddy, E., Weindel, M. Dong, S and Maraia, R. J. The fission yeast TFIIB-related factor, Brf1, limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment. Nucleic Acids Res., 31, 2108-2116 (2003)
  17. Huang, Y.* Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Nucleic Acids Res., 30, 1465-1482 (2002)
  18. Hamada, M., Huang, Y., Lowe, T. M. and Maraia, R. J. Widespread use of TATA elements in the core promoters for RNA polymerase III, II and I in fission yeast.  Mol. Cell. Biol., 21, 6870-6881 (2001)
  19. Huang, Y.*, Hamada, M., Patel, J. and Maraia, R. J. Construction of FLAG and histidine tagging vectors for Schizosaccharomyce pombe. Yeast, 18, 463-468 (2001)
  20. Huang, Y. and Maraia, R. J. Comparison of RNA polymerase III transcription machinery in Schizosaccharomyce  pombe, Saccharomyces cerevisiae and humans. Nucleic Acids Res., 29, 2675-2690 (2001)
  21. Huang, Y., Hamada, M. and Maraia, R. J. Isolation and cloning of four subunits of a fission Yeast TFIIIC Complex that includes an ortholog of the human regulatory protein TFIIIC. J. Biol. Chem., 275, 31480-31487 (2000)
  22. Intine, R., Sakulich, A., Koduru, S., Huang, Y., Pierstorff, E., Goodier, J., Phan, L. and Maraia, R. J. Control of transfer RNA maturation by phosphorylation of the human La antigen on serine 366. Mol. Cell, 6, 339-348 (2000)
  23. Huang, Y., Eckstein, F., Padilla, R., and Sousa, R. The mechanism of ribose 2’-group discrimination by an RNA polymerase.  Biochemistry, 36, 8231-8242 (1997)
  24. Huang, Y., Beaudry, A., McSwiggen, J. and Sousa, R. Determinants of ribose specificity in RNA polymerization: effects of Mn2 and dNMP incorporation into transcripts. Biochemistry, 36, 13718-28 (1997)
  25. Huang, Y., Garrison, P. N. and Barnes, L. D. Cloning of the Schizosaccharomyce pombe gene encoding diadenosine 5', 5'''-P1, P4-tetraphosphate asymmetrical hydrolase: sequence similarity with the histidine triad (HIT) protein family. Biochem. J. 1995, 312,925-932 (1995)

 

邀请国内外专家:   

1.  2007年,105年校庆期间邀请中科院院士王恩多来我校做学术访问,并作了题为“人氨基酰-tRNA 合成酶及相关tRNA的研究”的学术报告和一场主题为“事业与人生”的座谈会 。

2.  2008年,105年校庆期间邀请美国纽约大学教授Louis Levinger博士作学术报告: “Pre-tRNA 3' end processing and human disease”

3.  2008年,邀请加州大学柏克利分校分子与细胞生物学系教授,厦门大学生命科学学院闽江学者讲座教授、博士生导师周强教授作学术报告:"The Yin and Yang of P-TEFb regulation: Implications for HIV gene expression, cell growth control and cancer"

4.  2009年,邀请新加坡Institute of Molecular and Cell Biology, Wang Yue教授作学术报告: "Candida albicans hyphal development---from signal sensing to polarity control"

5.  2009年,邀请法国Ciarán Condon博士,Institut de Biologie Physico-Chimique, 作学术报告: "B. subtilis as an alternative bacterial model of RNA maturation and decay"

6. 在2012年我校110年校庆期间,邀请美国纽约大学的Louis Levinger教授来我校访问并做了题为“tRNase Z reaction is central to pre-tRNA maturation”的学术报告。

7. 2013年12月11日,邀请韩国the Korea Advanced Institute of Science and Technology (KAIST)的Park教授来我校访问并做“Structure and function of DJ-1 associated with Parkinson’s disease”的学术报告。

学术会议

  1. 2014年,The 25th tRNA Conference, 21-25 September 2014, Kyllini (West Peloponnese), Greece
  2. 2014年,第八届全国核糖核酸学术讨论会,合肥,安徽
  3. 2014年,The 10th International Mycological Congress,Bangkok,Thailand
  4. 2014年,中国生物化学与分子生物学会第十一次全国会员代表大会,厦门,福建
  5. 2014年,中国菌物学会第六届会员代表大会,贵阳,贵州
  6. 2014年,第七届中国模式真菌研讨会,南京,江苏
  7. 2013年,全国医学生化学术年会,青岛
  8. 2013年,第五届全国酵母生物学学术研讨会, 北京
  9. 2013年,中国细胞生物学学会2013年全国学术大会,武汉,湖北
  10. 2013 年,The 7th International Fission Yeast Meeting: Pombe2013, London, UK
  11. 2012年,华东六省一市生物化学与分子生物学学会2012年学术交流会,徐州
  12. 2012年,第四届全国微生物基因组学学术研讨会,济南,山东
  13. 2012年,冷泉港亚洲2012年表观遗传学、染色质与转录会议,苏州,江苏
  14. 2012年,The 22nd IUBMB & 37th FEBS Congress,Seville,Spain
  15. 2011年,International Union of Microbiological Societies (IUMS) 2011 Congress, Sapporo
  16. 2011年,亚太地区蛋白质学会第三届学术会议"暨"中英双边蛋白质科学研讨会,上海
  17. 2011年,首届哈尔滨国际沙门菌及其他肠道菌基因组学和生物学研讨会
  18. 2011年,2011年度RNA国际会议, Kyoto,Japan
  19. 2010年, 第六届全国核糖核酸(RNA)学术讨论会, 广州
  20. 2010年,第四届全国微生物遗传学学术研讨会, 杭州
  21. 2010年,中国生化与分子生物学会第十届全国学术会议,南京
  22. 2010年, 2010年度RNA国际会议, Seattle
  23. 2009年, 2009年度RNA国际会议, Wisconsin-Madison
  24. 2009年,第11届中国细胞生物学学术大会暨西安细胞生物学国际会议
  25. 2009年,东京第五届裂殖酵母会议, 西安
  26. 2009年, “The International Symposium on Advances in Modern Biology”, 本校
  27. 2009年, 2009年国际生化大会, 上海
  28. 2008年,亚洲-太平洋地区裂殖酵母学术讨论会, 新加坡
  29. 2007年,”第五届全国核糖核酸(RNA)学术讨论会,“与前列腺癌相关蛋白ELAC2/HsTRZ2同源、裂殖酵母tRNA 3’末端加工酶Trz1p功能的研究",上海
  30. Huang, Y., Bayfield, M. and Maraia. R. J. “Modular basis of a La protein activity in the maturation of structurally-impaired pre-tRNA that is distinguishable from 3' end protection” The Second East Coast Regional Fission Yeast Meeting, 2005, Miami, FL.,USA.
  31. Huang, Y., Bayfield, M. and Maraia. R. J. “Pre-tRNAs that require either one or two functions of La protein: protection from 3'-mediated decay by the nuclear exosome-Rrp6p, and facilitation of processing”. RNA 2005: the Tenth Annual Meeting of the RNA Society, Banff, Canada.
  32. Huang, Y. and Maraia, R. J. “Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3' oligo-U length and La-dependent tRNA processing in fission yeast”. 12th Biennial Meeting On Post-Initiation Activities of RNA Polymerases 2004, Mountain Lake, Virginia, USA
  33. Huang, Y., Intine, R., Hasson, S. and Maraia, R. J. “Mechanism of RNA 3' end formation by polymerase III Rpc11p in La-dependent tRNA processing”. NIH Research Festival 2004, Bethesda, MD,USA
  34. Huang, Y., Intine, R., Hasson, S. and Maraia, R. J. “Mechanism of RNA 3' end formation by polymerase III Rpc11p in La-dependent tRNA processing”. RNA Polymerase I & III    Transcription Conference, 2004, Pacific Grove, California,USA
  35. Huang, Y. and Maraia, R. J. “The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment”. The First East Coast Regional Fission Yeast Meeting, 2003, Worcester, MA,USA
  36. Huang, Y., McGillicuddy, E., Weindel, M. and Maraia, R. J.“RNA Polymerase III is limited to a TATA-dependent pathway of TBP recruitment in fission yeast”. The Enzymology of Chromatin and Transcription Keystone Symposium, 2003, Santa Fe, New Mexico,USA
  37. Huang, Y., Weindel, M., McGillicuddy, E. and Maraia, R. J. “Insufficiency of Brf1 as a stable TAF limits Polymerase III initiation complexes to a TATA-dependent pathway of TBP recruitment in S. pombe”. RNA Polymerase I & III Transcription Conference, 2002, Pacific Grove, California,USA
  38. Huang, Y., Hamada, M. and Maraia, R. J. “Isolation and characterization of TFIIIC from S. pombe: Identification of the yeast homolog of human TFIIICb, a key regulator of polymerase III transcription”. RNA Polymerase I & III Transcription Conference, 2000, Pacific Grove, California,USA
  39. Hamada, M., Huang, Y. and Maraia, R. J. “Transcription termination by RNA polymerase III in fission yeast: A genetic and biochemically-tractable model system”. 10th Biennial   Meeting On Post-Initiation Activities of RNA Polymerases 2000, Mountain Lake, Virginia,USA