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何凌峰

副教授,硕士生导师

电子邮件:lfhe22@139.com或者 08299@njnu.ac.cn

 

研究方向:

DNA损伤修复和肿瘤生物学机制

靶向DNA修复途径的小分子抑制剂

噬菌体展示与单克隆抗体药物研发

 

何凌峰博士,2009年毕业于中科院上海生命科学院(生化细胞所),获中国科学院博士学位(生化与分子生物学专业)。博士毕业后赴美国哈佛医学院Beth IsraelMedicalCenter做博士后研究(生物医学)。2013年回国并任职于南京师范大学生命科学学院。在读研究生和国外博士后工作期间,先后参加了国家863高科技计划(癌症的靶向基因病毒治疗研究和产品研发),中科院知识创新工程(肿瘤生物技术治疗的创新研究),以及美国NIH科研项目(SOX基因和肿瘤发生的分子机制)等科研项目。回国后先后主持了江苏省自然科学基金、教育部留学回国人员科研启动基金、南师大高层次人才科研启动项目、企业横向合作等科研项目。迄今已发表多篇研究论文,出版在Gene Medicine, Cancer LettersJBCOncogene, Cellular Signalling, EBioMedicine,Molecular Oncology,EMM,DNA Repair, Mol Can Ther等国际学术期刊。

 

教育背景和工作经历:

19909月-19948月:中国海洋大学,海洋生命科学院,海洋生物专业本科。

19949月-20038月:南京医药产业集团(中山制药),中成药研究,助理工程师。

20039月-200810月:中科院上海生命科学研究院(生化细胞所)硕博连读研究生。

20091月获中国科学院博士学位(生化与分子生物学专业)。

200811月-201212月:美国哈佛医学院,BethIsrael Medical Center,博士后。

20133月-至今:南京师范大学生命科学院,分子细胞生物学研究所,教师。

 

5年发表的学术论文:

(1) Ci S, Xia W, Liang W, Qin L, Zhang Y, Dianov GL, Wang M, Zhao X, Wu C, Alagamuthu KK, Hu Z,He L, Pan F, Guo Z.Src-mediated phosphorylation of GAPDH regulates its nuclear localization and cellular response to DNA damage.FASEBJ.2020 Jun 15. doi: 10.1096/fj.201902904RR. Online ahead of print. PMID:32539222.

 

(2) Zhu H, Wu C, Wu T, Xia W, Ci S, He W, Zhang Y, Li L, Zhou S, Zhang J, Edick AM, Zhang A, Pan FY, Hu Z, He L*(共同通讯作者), Guo Z*. Inhibition of AKT Sensitizes Cancer Cells to Antineoplastic Drugs by Downregulating Flap Endonuclease 1.Mol Cancer Ther. 2019 Aug 29.

doi: 10.1158/1535-7163.MCT-18-1215. [Epub ahead of print]PMID:31467180.

 

(3) Zhang J, Jing L, Li M, He L, Guo Z. Regulation of histone arginine methylation/demethylationby methylase and demethylase(Review). Mol Med Rep. 2019 May;19(5):3963-3971. doi: 10.3892/mmr.2019.10111. Epub 2019 Apr 1. Review.PMID:30942418.

 

(4) Lu X, Liu R, Wang M, Kumar AK, Pan F, He L, Hu Z, Guo Z. MicroRNA-140 impedes DNA repair by targeting FEN1 and enhances chemotherapeutic response in breast cancer.Oncogene. 2020 Jan;39(1):234-247. doi: 10.1038/s41388-019-0986-0.Epub 2019 Aug 30.PMID:31471584.

 

(5) Xia W, Ci S, Li M, Wang M, Dianov GL, Ma Z, Li L, Hua K, Alagamuthu KK, Qing L, Luo L, Edick AM, Liu L, Hu Z, He L, Pan F, Guo Z. Two-way crosstalk between BER and c-NHEJ repair pathway is mediated by Pol-β and Ku70.FASEB J. 2019 Nov;33(11):11668-11681.

doi:10.1096/fj.201900308R. Epub 2019 Jul 26.PMID:31348687.

 

(6) Wang M, Li E, Lin L, Kumar AK, Pan F, He L, Zhang J, Hu Z, Guo Z.Enhanced Activity of Variant DNA Polymerase β (D160G) Contributes to Cisplatin Therapy by Impeding the Efficiency of NER. Mol Cancer Res. 2019 Jul 26. doi: 10.1158/1541-7786. MCR-19-0482. [Epub ahead of print] PMID:31350308.

 

(7) Lingfeng He#, Hong Zhu#, Shiying Zhou#, Ting Wu#, Huan Wu, Huan Yang, Huiwen Mao, Chandra SekharKathera, Avilala Janardhan, Ashlin M. Edick, Anna Zhang, Zhigang Hu, Feiyan Pan and Zhigang Guo. Wnt pathway is involved in 5-FU drug resistance of colorectal cancer cells. Experimental & Molecular Medicine.2018 Aug 14;50(8):101. doi: 10.1038/s12276-018-0128-8.

 

(8) Lingfeng He#, Huan Yang#, Shiying Zhou#, Hong Zhu, Huiwen Mao, Zhuang Ma, Ting Wu,Alagamuthu Karthick Kumar, Chandrasekhar Kathera, Avilala Janardhan, Feiyan Pan, Zhigang Hu, Yanhua Yang, Libo Luo, Zhigang Guo. Synergistic antitumor effect of combined paclitaxel with FEN1 inhibitor in cervical cancer cells. DNA Repair. 2018 Mar; 63:1-9.

doi: 10.1016/j.dnarep.2018.01.003. Epub 2018 Jan 9.

 

(9) Janardhan A, Kathera C, Darsi A, Ali W, He L, Yang Y, Luo L, Guo Z. Prominent role of histone lysine demethylases in cancer epigenetics and therapy (Review).Oncotarget. 2018 Jan 25;9(76):34429-34448. doi:10.18632/oncotarget.24319. eCollection 2018 Sep 28. PMID:30344952.

 

(10) Lu X, He L, Zhou Q, Wang M, Shen WJ, Azhar S, Pan F, Guo Z, Hu Z. NHERF1 and NHERF2 regulation of SR-B1 stability via ubiquitination and proteasome degradation.BiochemBiophys Res Commun. 2017 Sep 2;490(4):1168-1175. doi: 10.1016/j.bbrc.2017.06.175.

Epub 2017 Jun 29. PMID:28669731.

 

(11) He L#, Luo L#, Zhu H, Yang H, Zhang Y, Wu H, Sun H, Jiang F, Kathera CS, Liu L, Zhuang Z, Chen H, Pan F, Hu Z, Zhang J, Guo Z. FEN1 Promotes Tumor Progress and Confers Cisplatin Resistance in Non-Small Cell Lung Cancer. Molecular Oncology. 2017 Jun;11(6):640-654.

doi: 10.1002/1878-0261.12058. Epub 2017 May 12.

 

(12) Kathera C, Zhang J, Janardhan A, Sun H, Ali W, Zhou X, He L, Guo Z. Interacting partners of FEN1 and its role in the development of anticancer therapeutics (Review).Oncotarget. 2017 Apr 18;8(16):27593-27602. doi: 10.18632/oncotarget.15176. PMID: 28187440

(13) Hongfang Sun#,Lingfeng He#Co-First author, Huan Wu#, Feiyan Pan, Xuping Wu, Jing Zhao, ZhigangHu,Chandra Sandra, Binghui Shen, Zhigang Guo. The FEN1 L209P mutation interferes with long patch base excision repair and induces cellular transformation. Oncogene 2017 Jan 12;36(2):194-207. doi: 10.1038/onc.2016.188.Epub 2016 Jun 6.

 

(14) Lingfeng He#, Yilan Zhang#, Hongfang Sun#, Feng Jiang, Huan Yang, Huan Wu, Ting Zhou, Sencai Hu, Chandra Sekhar Kathera, Xiaojun Wang, Haoyan Chen, Hongzhi Li, Binghui Shen, Yongqiang Zhu, Zhigang Guo. Targeting DNA Flap Endonuclease 1 to impede breast cancer progression.EBioMedicine.2016 Dec;14:32-43. doi: 10.1016/j.ebiom.2016.11.012. Epub 2016 Nov 10.

 

(15) Xiaolong Zhou#, Ziheng Zhuang#, Wentao Wang#, Lingfeng He#Co-First author, Huan Wu, Yan Cao, Feiyan Pan, Jing Zhao, Zhigang Hu, Chandra Sekhar, Zhigang Guo. OGG1 is essential in oxidative stress induced DNA demethylation. Cell Signal. 2016 Sep;28(9):1163-71. doi: 10.1016/j.cellsig.2016.05.021. Epub 2016 May 29.

 

(16) Feiyan Pan#, Jing Zhao#, Ting Zhou, ZhihuiKuang, Huifang Dai, Huan Wu,Hongfang Sun, Xiaolong Zhou, Xuping Wu, Zhigang Hu,Lingfeng He, Binghui Shen &Zhigang Guo. Mutation of DNA Polymeraseβ R137Q Results in Retarded Embryo Development Due to Impaired DNABase Excision Repair in Mice. Sci Rep. 2016 Jun 30;6:28614. doi: 10.1038/srep28614.

 

(17) Ma F, Ye H, He HH, Gerrin SJ, Chen S, Tanenbaum BA, Cai C, Sowalsky AG, He L, Wang H, Balk SP, Yuan X. SOX9 drives WNT pathway activation in prostate cancer.J Clin Invest. 2016 May 2;126(5):1745-58. doi: 10.1172/JCI78815. Epub 2016 Apr 4.

 

(18) Ting Zhou#, Feiyan Pan #, Yan Cao, Ying Han, Jing Zhao, Hongfang Sun, Xiaolong Zhou, Xuping Wu, Lingfeng He, Zhigang Hu, Haoyan Chen, Binghui Shen, Zhigang Guo. R152C DNA Pol β mutation impairs base excision repair and induces cellular transformation.Oncotarget. 2016 Feb 9;7(6):6902-15.doi: 10.18632/oncotarget.6849. PMID: 26760506.

 

(19) Yan Cao#, Huan Yang#, Xiaolong Zhou, Huiwen Mao, Tingting Gao, Zhigang Hu, Lingfeng He, Feiyan Pan, Zhigang Guo. Selection and characterization of human PCSK9 antibody from phage displayed antibody library. BiochemBiophys Res Commun. 2015 Aug 7;463(4):712-8. doi: 10.1016/j.bbrc.2015.05.129. Epub 2015 Jun 5.PMID:26056005.

 

(20) 周婷,高婷婷,孙洪芳,潘飞燕,何凌峰,郭志刚。结构特异性核酸内切酶FEN1及其与肿瘤的关系,《南京师范大学学报》,第37 卷第2 期2014 年6 月。