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郭志刚

郭志刚

教授、博士生导师、南京师范大学生命科学学院副院长

通讯地址:江苏省南京市文苑路1号,邮编:210023
E-mail: guo@njnu.edu.cn
 

基本情况

2005年毕业于南京大学,获生物化学与分子生物学博士学位。毕业后加入美国Tanox生物制药公司从事抗体药物研发。之后赴美国希望城国家医学中心从事DNA修复与抗肿瘤研究。20113月入职南京师范大学,受聘教授、博士生导师。现任南京师范大学生命科学学院副院长、江苏省生物化与分子生物学学会常务理事兼青年委员会主任。

 

人才称号

科技部国家重大发展计划青年科学家专题(青年973计划)首席科学家(2013

教育部新世纪优秀人才(2014

江苏省双创人才(2011

江苏省杰出青年基金获得者(2013

南京师范大学百名青年领军人才(2014

江苏省六大人才高峰计划(2015

江苏省333工程第三层次(2014

 

荣誉或奖励

第五届国家侨界创新贡献奖(2014

14届江苏青年科技奖(2014

11届南京市自然科学优秀论文一等奖(2013

江苏省科协青年创业大赛二等奖(2016

南京师范大学优秀教师奖(2012

 

学术兼职

美国科学促进会、美国肿瘤研究会、美国Sigma科学研究会会员、中国细胞生物学会医学细胞生物学分会理事、江苏省发育与细胞生物学会理事、江苏省生物化学与分子生物学学会常务理事兼青年委员会主任。

 

主要研究方向

1 DNA损伤修复与抗肿瘤研究

2)抗体工程及治疗性抗体的开发与应用

 

承担的主要课题:

主持国家科技部青年973计划1项、国家自然科学面上项目1项、教育部新世纪优秀人才支持计划1项、教育部博士点基金1个(博导类)、江苏省杰出青年基金1项、江苏科技厅重大研究项目1项,江苏省双创计划项目1项、其它部省级、横向课题多项,总经费1000多万元。

 

发表论文:
 

  1. Lingfeng He, Hong Zhu, Huan Yang, Huan Wu, Hongfang Sun, Feng Jiang, Chandra Sekhar Kathera, Yilan Zhang1, Haoyan Chen, Feiyan Pan, Zhigang Hu, Zhigang Guo1(通讯作者)*. FEN1 Promotes Tumor Progress and Confers Cisplatin Resistance in non-small cell Lung Cancer. Molecular Oncology 2017 (under review).

 

  1. Chandrasekhar kathera, Hongfang Sun, Wajid Ali, Xiaolong Zhou, Lingfeng He, Zhigang Guo(通讯作者)*. Interacting partners of FEN1 and its role in the development of anticancer therapeutics. Oncotarget 2017 (accepted)

 

  1. Xuping Wu, Jing Zhang2, Sijun Yang , Zhihui Kuang, Guolei Tan, Gang Yang, Qichun Wei, Zhigang Guo(共同通讯作者)*.Telomerase antagonist imetelstat increases radiation sensitivity in esophageal squamous cell carcinoma. Oncotarget 2017 (online published)

 

  1. Lingfeng He a , Yilan Zhang , Hongfang Sun, Feng Jiang, Huan Yang , Huan Wu , Ting Zhou , Sencai Hu , Chandra Sekhar Kathera , Xiaojun Wang , Haoyan Chen , Hongzhi Li, Binghui Shen, Yongqiang Zhu , Zhigang Guo(通讯作者)*. Targeting DNA Flap Endonuclease 1 to Impede Breast Cancer Progression. EBioMedicine 14 (2016) 32–43.

 

  1. Pan, F., Jing Zhao, Ting Zhou, Zhihui Kuang, Huifang Dai, Huan Wu, Hongfang Sun, Xiaolong Zhou, Xuping Wu, Zhigang Hu, Lingfeng He, Binghui shen, Zhigang Guo(通讯作者)*. Mutation of DNA Polymerase β R137Q Results in Retarded Embryo Development Due to Impaired DNA Base Excision Repair in Mice. Scientific Report, 2016. 6: p. 28614.

 

  1. Ting Zhou,Yan Cao, Ying Han, Jing Zhao, Hongfang Sun,Xiaolong Zhou, Xuping Wu,Lingfeng He, Zhigang Hu,Haoyan Chen*, Binghui shen*, Zhigang Guo(通讯作者)*. The DNA Pol β R152C Mutation Impairs Base Excision Repair and Induces Cellular Transformation. Oncotarget 2016 Feb 9;7(6):6902-15. doi: 10.18632/oncotarget.6849.

 

  1. Hongfang Sun, Lingfeng He, Huan Wu, Feiyan Pan, Xuping Wu, Jing Zhao, Zhigang Hu, Chandra Sekhar, Hongzhi Li, Li Zheng, Haoyan Chen, Binghui Shen, Zhigang Guo(通讯作者)*. The FEN1 L209P mutation interferes with long patch base excision repair and induces cellular transformation. Oncogene. 2016 Jun 6. doi: 10.1038/onc.2016.188.

 

  1. Zhou X, Zhuang Z, Wang W, He L, Wu H, Cao Y, Pan F, Zhao J, Hu Z, Sekhar C, Zhigang Guo *. OGG1 is essential in oxidative stress induced DNA demethylation. Cell Signal. 2016 Sep;28(9):1163-71. doi: 10.1016/j.cellsig.2016.05.021. Epub 2016 May 29.

 

  1. Hu Zhigang*, Li Jiaxin, Kuang Zhihui, Wang Meina, Azhar Salman, and Zhigang Guo(共同通讯作者)*.  Polymorphism and Hormonal Regulation of DNA Methylation in Scavenger Receptor Class B, Type I. DNA and Cell Biology. June 2016, 35(6): 280-289. doi:10.1089/dna.2015.3185. 

 

  1. Cao Y, Yang H, Zhou X, Mao H, Gao T, Hu Z, He L, Pan F, Guo Z(通讯作者)*. Selection and characterization of human PCSK9 antibody from phage displayed antibody library. Biochem Biophys Res Commun. 2015 Aug 7;463(4):712-8.
  2. L Chung, D Onyango, Guo Z, P Jia, H Dai, S Liu, M Zhou, W Lin, L Zheng, J Lopes, A Nicolas, W Chai, D Raz, KL Reckamp and B Shen. The FEN1 E359K germline mutation disrupts the FEN1–WRN interaction and FEN1 GEN activity, causing aneuploidy-associated cancers. Oncogene (2015) 34, 902–911

 

  1. GUO Z, Julie Kanjanapangka, Na Liu, Songbai Liu, Changwei Liu, Zhenxing Wu,Yingjie Wang, Tiffany Loh, Claudia Kowolik, Joonas Jamsen,  Mian Zhou, Khue Truong,  Yuan Chen, Li Zheng, Binghui Shen. Sequential Posttranslational Modifications Program FEN1 Degradation during Cell-Cycle Progression. Molecular Cell (2012) 47, 444–456.  

 

  1. Li Zheng, Huifang Dai, Mian Zhou, Xiaojin Li, Changwei Liu, Guo Z, Xiwei Wu, Jun Wu, Charles Wang, John Zhong, Qin Huang, Julio Garcia-Aguilar, Gerd P. Pfeifer, Binghui Shen.  Polyploid cells rewire DNA damage response networks to overcome replication stress-induced barriers for tumour progression Nature Communications(2012)3 ,doi:10.1038/ncomms1825

 

  1. Li Zheng, Jia Jia, L. David Finger, Guo Z, Cindy Zer, and Binghui Shen. Functional regulation of FEN1 nuclease and its link to cancer Nucleic Acids Res. 2011 Feb; 39(3): 781–794.

 

  1. Li Zheng, Huifang Dai, Muralidhar L Hegde, Mian Zhou, Zhigang Guo, Xiwei Wu, Jun Wu, Lei Su, Xueyan Zhong, Sankar Mitra, Qin Huang, Kemp H Kernstine, Gerd P Pfeifer and Binghui Shen. Fen1 mutations that specifically disrupt its interaction with PCNA cause aneuploidy-associated cancer. Cell Research (2011) 21:1052-1067. 

 

  1. Guo, Z.Li Zheng, Hong Xu, Huifang Dai, Mian Zhou, Mary Rose Pascua, Qin M. Chen, and Binghui Shen 2010. Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding. Nature Chemical Biology (2010) 6: 766-773. 

 

  1. Guo, Z., Z. Li, H. Dai, M. Zhou, H. Xu, and B. Shen. Human DNA Pol β polymorphism, Arg137Gln, impairs Pol β polymerase activity, interaction with PCNA, and base excision repair capacity. Nucleic Acids Research (2009) 37: 3431-3441

 

  1. Li Zheng, Mian Zhou, Guo, Z, Huiming Lu, Limin Qian, Huifang Dai, Junzhuan Qiu, Elena Yakubovskaya, Daniel F. Bogenhagen, Bruce Demple, Binghui Shen. Human DNA2 Is a Mitochondrial Nuclease/Helicase for Efficient Processing of DNA Replication and Repair Intermediates Molecular cell (2008) 32(3):325–336

 

  1. Guo, Z., V. Chavez, P. Singh, L. D. Finger, H. Hang, M. L. Hegde, and B. Shen.  Comprehensive mapping of the C-terminus of flap endonuclease-1 reveals distinct interaction sites for five proteins that represent different DNA replication and repair pathways. Journal of Molecular Biology (2008) 377:679-90. 

 

  1. Guo, Z., L. Qian, R. Liu, H. Dai, M. Zhou, L. Zheng, and B. Shen.  Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair. Molecular and Cellular Biology  (2008) 28:4310-9.

 

  1. Physical and Functional Interaction between Human Oxidized Base-specific DNA Glycosylase NEIL1 and Flap Endonuclease 1 Muralidhar L. Hegde, Corey A. Theriot, Aditi Das, Pavana M. Hegde, Zhigang Guo,Ronald K. Gary ,Binghui Shen and Sankar Mitra. The Journal of Biological Chemistry,283,27028-27037.

 

  1. Guo Z1, Bi FTang YZhang JYuan DXia ZLiu JN. Preparation and characterization of scFv for affinity purification of reteplase. J Biochem Biophys Methods. 2006 Apr 30;67(1):27-36. Epub 2006 Feb 10.

 

  1. Chen QianJian-Ning LiuFengyuan TangDawen YuanZhigang Guo and Jing Zhang.  A Novel Strategy for Proteome-wide Ligand Screening Using Cross-linked Phage Matrices. Journal of Biological Chemistry (2008) 285,9367-9372.

 

  1. Ying-Jiang Zhou, Shi-Quan Wang, Jing Zhang, Wei Zhang, Feng Bi, Zhi-Gang Guo, Bi-Sen Ding, Pat Kumar, Jian-Ning Liu, Xiang-Yang Tan A novel method to isolate and map endothelial membrane proteins from pulmonary vasculature. American Journal of Physiology - Cell Physiology ( 2005) 288(4) C950-C956 

 

  1. Wei Zhang, Ying-Jiang Zhou, Zhi-Gang Guo, Feng Bi, Jing Zhang, Pat Kumar, Xiang-Yang Tan, and Jian-Ning Liu. Preparation of Tissue-Specific Monoclonal Antibodies Using Purified Endothelial Membrane Proteins from Biotinylated Pulmonary Vasculature of Rhesus Monkey. Hybridoma (2006),