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戴亦军

 

戴亦军,男,1972年生,博士,教授,博士生导师,生命科学学院生物技术专业主任,微生物生物技术研究所副所长。

主要研究方向为微生物生物技术,目前从事微生物资源生物技术和微生物降解有机污染物研究,研究内容涉及功能微生物的筛选和鉴定、污染物降解途径分析、代谢酶的基因克隆表达、酶学特性及转录调控机制。主持了4项国家自然科学基金面上项目以及环保公益性行业专项、江苏省自然科学基金和江苏省高校自然科学研究重大项目等多个课题;发表SCI论文30余篇,获授权中国发明专利8项。

在教育教学方面,目前担任微生物学实验课程校级教学团队主持人,主持江苏省在线开放课程《微生物学模块化实验》建设与线上教学,主持校课程思政教改项目1项。为本科生讲授了《发酵工程》、《微生物学实验》和《生命科学导论》等课程。曾获2010年度江苏省高校“青蓝工程”优秀青年骨干教师。

一、主要科研项目

低营养诱导粘着箭菌啶虫脒腈水合酶基因pnhA表达的转录调控机制研究,国家自然科学基金面上项目, 2020.01-2023.12

细菌代谢吡虫啉的羟基化和硝基还原途径中的关键酶和调节机制研究, 国家自然科学基金面上项目, 2016.01-2019.12

微生物共代谢降解烟碱类杀虫剂吡虫啉的关键酶及其调控机制研究,国家自然科学基金面上项目,2014.01-2014.12

不同共代谢基质控制嗜麦芽寡养单胞菌的吡虫啉代谢途径的机理研究,国家自然科学基金面上项目,2010.01-2012.12

二、发表论文(*,corresponding author)

()主要研究论文

Yang WL, Dai ZL, Guo L, Fan ZX, Ge F*, Dai YJ* (2020) Sulfoxaflor degraded by Aminobacter sp. CGMCC 1.17253 through hydration pathway mediated by nitrile hydratase. Journal of Agricultural and Food Chemistry,68(16): 4579-4587

Wang YX, Wang G, Dai YJ*, Wang Y,Lee YW, Shi JR* and Xu JH* (2020) Biodegradation of deoxynivalenol by a novel microbial consortium.Frontiers in Microbiology, 10:2964.doi: 10.3389/fmicb.2019.02964

Guo LL, Dai ZL, Guo JJ, Yang WL, Ge F*, Dai Yijun*(2020) Oligotrophic bacteriumHymenobacterlatericoloratus CGMCC 16346 degrades the neonicotinoid imidacloprid in surface water, AMB Express,10: 7. doi: 10.1186/s13568-019-0942-y

Yang WL, Guo LL, Dai ZL, Q RC, Zhao YX, and Dai YJ* (2019) Biodegradation of the insecticide flonicamid by Alcaligenes faecalisCGMCC 17553 via hydrolysis and hydration pathways mediated by nitrilase. Journal of Agricultural and Food Chemistry,67(36): 10032−10041

Guo L, Fang WW, Guo LL, Yao CF, Zhao YX, Ge F*, Dai YJ* (2019) Biodegradation of the neonicotinoid insecticide acetamiprid by actinomycetes Streptomyces canus CGMCC 13662 and characterization of the novel nitrile hydratase involved. Journal of Agricultural and Food Chemistry,67(21): 5922-5931

Sun SL, Fan ZX, Zhao YX, Guo LL, Dai YJ*(2019) A novel nutrient deprivation-induced neonicotinoid insecticide acetamiprid degradation by Ensifer adhaerens CGMCC 6315. Journal of Agricultural and Food Chemistry,67(1): 63-71

Zhao YX, Jiang HY, Chen X, Zhu YX, Fan ZX, Dai ZL, Guo L, Liu ZH*, Dai YJ* (2019) Neonicotinoid thiacloprid transformation by the N2-fixing bacterium Microvirga flocculans CGMCC 1.16731 and toxicity of the amide metabolite. International Biodeterioration & Biodegradation, 145 (11): 104806

Zhao YX, Guo LL, Sun SL, Guo JJ,Dai YJ* (2019) Bioconversion of indole-3-acetonitrile by the N2-fixing bacteriumEnsifer meliloti CGMCC 7333 and its Escherichia coli-expressednitrile hydratase. International Microbiology, https://doi.org/10.1007/s10123-019-00094-0

Sun SL, Yang WL, Fang WW, Zhao YX, Guo L, Dai YJ*(2018) The plant growthpromoting rhizobacterium Variovorax boronicumulans CGMCC 4969 regulates the level of indole-3-acetic acid synthesized from indole-3-acetonitrile. Applied and Environmental Microbiology, 84(16):e00298-18

Sun SL, Yang WL, Guo JJ, Zhou YN, Rui X, Chen C, Ge F*, Dai YJ* (2017) Biodegradation of the neonicotinoid insecticide acetamiprid in surface water by the bacterium Variovorax boronicumulans CGMCC 4969 and its enzymatic mechanism. RSC Advances,7 (41): 25387–25397

Lu TQ, Mao SY, Sun SL, Yang WL, Ge F*, Dai YJ* (2016) Regulation of hydroxylation and nitroreduction pathways during metabolism of the neonicotinoid insecticide imidacloprid byPseudomonas putida. Journal of Agricultural and Food Chemistry, 64 (24): 4866–4875

Sun SL, Lu TQ, Yang WL, Guo JJ, Rui X, Mao SY, Zhou LY, Dai YJ* (2016) Characterization of a versatile nitrile hydratase of the neonicotinoid thiacloprid-degrading bacterium Ensifer meliloti CGMCC 7333.RSC Advances,6 (19):15501–15508

Zhou LY, Zhang LJ*, Sun SL, Ge F, Mao SY, Ma Y, Liu ZH, Dai YJ*, Yuan S (2014) Degradation of the neonicotinoid insecticide acetamiprid via the N-carbamoylimine derivate (IM-1-2) mediated by the nitrile hydratase of the nitrogen-fixing bacterium Ensifer meliloti CGMCC 7333. Journal of Agricultural and Food Chemistry,62(41): 9957-9964

Ge F, Zhou LY, Wang Y, Ma Y, Zhai S, Liu ZH, Dai YJ*, Yuan S (2014) Hydrolysis of the neonicotinoid insecticide thiacloprid by the N2-fixing bacterium Ensifer meliloti CGMCC 7333. International Biodeterioration & Biodegradation, 93:10-17

Ma Y, Zhai S, Mao SY, Sun SL, Wang Y, Liu ZH, Dai YJ*, Yuan S (2014) Co-metabolic transformation of the neonicotinoid insecticide imidacloprid by the new soil isolate Pseudoxanthomonas indicaCGMCC 6648. Journal of Environmental Science and Health, Part B:Pesticides, Food Contaminants, and Agricultural Wastes, 49(9): 661-670

Zhou GC, Wang Y, Ma Y, Zhai S, Zhou LY, Dai YJ*, Yuan S (2014) The metabolism of neonicotinoid insecticide thiamethoxam by soil enrichment cultures, and the bacterial diversity and plant growth promoting properties of the cultured isolates.Journal of Environmental Science and Health, Part B:Pesticides, Food Contaminants, and Agricultural Wastes, 49(6): 381-390

Zhou GC, Wang Y, Zhai S, Ge F, Liu ZH, Dai YJ*, Yuan S, Hou JY (2013)Biodegradation of the neonicotinoid insecticide thiamethoxam by the nitrogen-fixing and plant-growth-promoting rhizobacterium Ensiferadhaerens strain TMX-23. Applied Microbiology and Biotechnology,97(9): 40654074

Liu ZH, Cao YM, Zhou QW, Guo K, Ge F*, Hou JY, Hu SY, Yuan S, Dai YJ* (2013) Acrylamide biodegradation ability and plant growth-promoting properties of Variovorax boronicumulans CGMCC 4969. Biodegradation,24:855–864

Liu ZH, Dai YJ*, Huan Y, Liu ZX, Sun Lei, Zhou QW, Zhang WJ, Sang Q, Wei H, Yuan S* (2013) Different utilizable substrates have different effects on cometabolic fate of imidacloprid in Stenotrophomonas maltophilia. Applied Microbiology and Biotechnology, 97:6537–6547

Liu ZH, Cao YM, Dai YJ, Ni JP, Wei H, Yuan S (2013) Pretreatment with Stenotrophomonas maltophilia CGMCC 1.1788 increased the aphicidal activity of imidacloprid. Journal of Pesticide Science,38(3): 139–143

Zhang HJ, Zhou QW, Zhou GC, Cao YM,Dai YJ*, Ji WW, Shang GD, Yuan S* (2012) Biotransformation of the neonicotinoid insecticide thiacloprid by bacterium Variovorax boronicumulans strain J1 and mediation of the major metabolic pathway by nitrile hydratase. Journal of Agricultural and Food Chemistry,60 (1):153–159

Liu ZH, Dai YJ*, Huang GD, Gu YY, Ni JP, Wei H, Yuan S* (2011) Soil microbial degradation of neonicotinoid insecticides imidacloprid, acetamiprid, thiacloprid and imidaclothiz and its effect on the persistence of bioefficacy against horsebean aphid Aphis craccivora Koch after soil application. Pest Management Science, 67 (10):1245-1252

Dai YJ, Zhao YJ, Zhang WJ, Yu CG, Ji WW, Xu WP, Ni JP, Yuan S*(2010) Biotransformation of thianicotinyl neonicotinoid insecticides: Diverse molecular substituents response to metabolism by bacterium Stenotrophomonas maltophilia CGMCC 1.1788. Bioresource Technology,101:3838-3843

Dai YJ, Ji WW, Chen T, Zhang WJ, Liu ZH, Ge F, Yuan S*(2010) Metabolism of the neonicotinoid insecticides acetamiprid and thiacloprid by the yeast Rhodotorula mucilaginosa Strain IM-2. Journal of Agricultural and Food Chemistry,58:2419–2425

Zhao YJ, Dai YJ, Yu CG, Luo J, Xu WP, Ni JP Yuan S* (2009) Hydroxylation of thiacloprid by bacterium Stenotrophomonas maltophilia CGMCC1.1788. Biodegradation, 20: 761-768

Chen T, Dai YJ (co-first author), Ding JF, Yuan S*, Ni JP (2008)N-demethylation of neonicotinoid insecticide acetamiprid by bacterium Stenotrophomonas maltophilia CGMCC 1.1788. Biodegradation,19:651-658

Dai YJ, Chen T, Ge F, Huan Y, Yuan S*, Zhu FF (2007) Enhanced hydroxylation of imidacloprid by Stenotrophomonas maltophilia upon addition of sucrose. Applied Microbiology and Biotechnology,74:995-1000

Dai YJ, Yuan S*, Ge F, Chen T, Xu SC, Ni JP (2006) Microbial hydroxylation of imidacloprid for synthesis of highly insecticidal olefin imidacloprid. Applied Microbiology and Biotechnology,71:927-934

(二)教改论文

何伟, 刘中华, 贾永, 张石柱, 韩管助, 许凯, 戴亦军*, 袁生. 综合性、研究型微生物学实验课的过程性考核. 微生物学通报,2020, 47(4): 1218−1223

戴亦军, 何伟, 袁生*, 刘中华, 贾永,韩管助. “互联网+”背景下微生物学实验课程的改革与实践. 微生物学通报, 2018, 45(3): 683690 (荣获2018年度微生物学通报优秀论文奖)

魏华,梅艳珍,戴亦军*,袁生.生物发酵制品实训课程教学模式探讨.酿酒, 2018, 45(2): 11-15

戴亦军, 何伟, 袁生*, 刘中华, 贾永, 张石柱. 研究型模块实验产淀粉酶菌株的筛选、发酵条件优化、菌种鉴定和选育的教学实践. 微生物学通报, 2017,44(4):976-982

戴亦军, 何伟, 袁生*, 徐旭士, 戴传超. 水质微生物学检验实验模块的教学探索与实践. 微生物学通报, 2016, 43(1): 211216

梅艳珍,魏华,戴亦军*,戴传超,袁生.建设啤酒生产实训课程,提高理科高校本科生综合实践能力.大学教育, 2016, 59(11):136-139

戴亦军, 何伟, 袁生*, 徐旭士, 贾永, 刘中华, 张石柱, 梅艳珍. 模块化微生物学实验课教学体系的探索与实践. 微生物学通报, 2015, 42(9): 18091816

戴亦军, 徐旭士, 袁生, 何伟, 贾永, 梅艳珍, 张石柱, 刘中华. 新课标视角下的高师微生物实验课程探究性教学研究. 大学教育, 2015, 58 (4): 139−141

 

通讯地址:江苏省南京市仙林大学城文苑路1号南京师范大学生命科学学院

邮政编码:210023

E-mail: daiyijun@njnu.edu.cn